CKM ELISA Kit (Rabbit) (OKCD01481)

Catalog No: OKCD01481 (Formerly GWB-KBBJW3)

Size:96 Wells

Rabbit CKM ELISA Kit Standard Curve

Product Info

• Predicted Species Reactivity: Rabbit
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~3.5 Hours
• ELISA Kit Linearity:

  Sample	1:2 Dilution	1:4 Dilution	1:8 Dilution	1:16 Dilution
  Serum (n=5)	90-98%	96-104%	92-101%	83-97%
  EDTA Plasma (n=5)	96-105%	83-97%	91-103%	79-101%
  Heparin Plasma (n=5)	79-94%	99-105%	97-104%	95-105%

• ELISA Kit Principle: Aviva Systems Biology CKM ELISA Kit (Rabbit) (OKCD01481) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CKM has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CKM is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution.The density of yellow coloration is read by absorbance at 450 nm and is quantitatively proportional to the amount of sample CKM captured in the well.
• ELISA Kit Range: 1.56 - 100 ng/mL
• ELISA Kit Recovery:

  Matrix	Recovery Range (%)	Average (%)
  Serum (n=5)	80-99	80
  EDTA Plasma (n=5)	93-104	97
  Heparin Plasma (n=5)	97-105	101

• ELISA Kit Reproducibility: Intra-assay Precision: 3 samples with known low, middle and high level CKM was tested with 20 replicates across one plate.
  Inter-assay Precision: 3 samples with known low, middle and high level CKM was tested on three different plates with 8 replicates, respectively.
  Intra-Assay CV: <10%
  Inter-Assay CV: <12%
• ELISA Kit Component:
  • Anti-CKM Microplate: 96 Wells (12 x 8 Well Strips)
  • CKM Lyophilized Standard: 2 x 100 ng
  • 100X Biotinylated CKM Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, plasma and other biological fluids
• Sensitivity: < 0.57 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Creatine Kinase, Muscle (CKM). No significant cross-reactivity or interference between Creatine Kinase, Muscle (CKM) and analogues was observed.
• Publications:

  Creatine kinase/a-crystallin interaction functions in cataract development. Biochem Biophys Rep. 22, 100748 (2020). 32154391

• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: CKM
• Alias Symbols: CPK-M
• NCBI Gene Id: 100009056
• Protein Name: Creatine kinase M-type
• Description of Target: Reversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate). Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa.
• Uniprot ID: P00563
• Protein Accession #: NP_001075708.1
• Nucleotide Accession #: NM_001082239.1
• Protein Size (# AA): 381