CKM ELISA Kit (Rabbit) (OKCD01481)

Catalog No: OKCD01481 (Formerly GWB-KBBJW3)

Size:96WELLS

Rabbit CKM ELISA Kit Standard Curve

Product Info

• Predicted Species Reactivity: Oryctolagus cuniculus|Rabbit
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2 Dilution	1:4 Dilution	1:8 Dilution	1:16 Dilution
  Serum (n=5)	90-98%	96-104%	92-101%	83-97%
  EDTA Plasma (n=5)	96-105%	83-97%	91-103%	79-101%
  Heparin Plasma (n=5)	79-94%	99-105%	97-104%	95-105%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Creatine Kinase, Muscle (CKM). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Creatine Kinase, Muscle (CKM). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Creatine Kinase, Muscle (CKM), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Creatine Kinase, Muscle (CKM) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 1.56-100ng/mL
• ELISA Kit Recovery:

  Matrix	Recovery Range (%)	Average (%)
  Serum (n=5)	80-99	80
  EDTA Plasma (n=5)	93-104	97
  Heparin Plasma (n=5)	97-105	101

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Creatine Kinase, Muscle (CKM) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Creatine Kinase, Muscle (CKM) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-CKM Microplate: 96 Wells (12 x 8 Well Strips)
  • CKM Lyophilized Standard: 2 x 100 ng
  • 100X Biotinylated CKM Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma and other biological fluids
• Sensitivity: < 0.57 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Creatine Kinase, Muscle (CKM). No significant cross-reactivity or interference between Creatine Kinase, Muscle (CKM) and analogues was observed.
• Publications:

  Creatine kinase/a-crystallin interaction functions in cataract development. Biochem Biophys Rep. 22, 100748 (2020). 32154391

• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay
• Description:
  View product citations for CKM ELISA kit (Rabbit) on CiteAb

Target Info

• Gene Symbol: CKM
• Gene Full Name: creatine kinase, M-type
• Alias Symbols: CPK-M;creatine kinase M chain;creatine kinase M-type;creatine kinase, muscle;creatine phosphokinase M-type;M-CK;muscle creatine kinase.
• NCBI Gene Id: 100009056
• Protein Name: Creatine kinase M-type
• Description of Target: Reversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate). Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa.
• Uniprot ID: P00563
• Protein Accession #: NP_001075708.1
• Nucleotide Accession #: NM_001082239.1
• Protein Size (# AA): 381