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Catalog No: OKCD01481 (Formerly GWB-KBBJW3)
Size:96WELLS
Price: $650.00
SKU
OKCD01481
Availability: Domestic: within 1-2 weeks delivery | International: within 1-2 weeks delivery
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Datasheets/ManualsPrintable datasheet for CKM ELISA Kit (Rabbit) (OKCD01481)
Product Info
Predicted Species ReactivityOryctolagus cuniculus|Rabbit
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit Linearity
Sample1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
Serum (n=5)90-98%96-104%92-101%83-97%
EDTA Plasma (n=5)96-105%83-97%91-103%79-101%
Heparin Plasma (n=5)79-94%99-105%97-104%95-105%
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Creatine Kinase, Muscle (CKM). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Creatine Kinase, Muscle (CKM). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Creatine Kinase, Muscle (CKM), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Creatine Kinase, Muscle (CKM) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range1.56-100ng/mL
ELISA Kit Recovery
MatrixRecovery Range (%)Average (%)
Serum (n=5)80-9980
EDTA Plasma (n=5)93-10497
Heparin Plasma (n=5)97-105101
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Creatine Kinase, Muscle (CKM) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Creatine Kinase, Muscle (CKM) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-CKM Microplate96 Wells (12 x 8 Well Strips)
CKM Lyophilized Standard2 x 100 ng
100X Biotinylated CKM Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma and other biological fluids
Sensitivity< 0.57 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Creatine Kinase, Muscle (CKM). No significant cross-reactivity or interference between Creatine Kinase, Muscle (CKM) and analogues was observed.
Publications

Creatine kinase/a-crystallin interaction functions in cataract development. Biochem Biophys Rep. 22, 100748 (2020). 32154391

Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Description
Gene SymbolCKM
Gene Full Namecreatine kinase, M-type
Alias SymbolsCPK-M;creatine kinase M chain;creatine kinase M-type;creatine kinase, muscle;creatine phosphokinase M-type;M-CK;muscle creatine kinase.
NCBI Gene Id100009056
Protein NameCreatine kinase M-type
Description of TargetReversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate). Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa.
Uniprot IDP00563
Protein Accession #NP_001075708.1
Nucleotide Accession #NM_001082239.1
Protein Size (# AA)381
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