Catalog No: OKCD01481 (Formerly GWB-KBBJW3)
Size:96 Wells
Price: $650.00
Availability: Domestic: within 2-3 weeks delivery | International: 2-3 weeks
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityRabbit
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~3.5 Hours
ELISA Kit Linearity
Sample1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
Serum (n=5)90-98%96-104%92-101%83-97%
EDTA Plasma (n=5)96-105%83-97%91-103%79-101%
Heparin Plasma (n=5)79-94%99-105%97-104%95-105%
ELISA Kit PrincipleAviva Systems Biology CKM ELISA Kit (Rabbit) (OKCD01481) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CKM has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CKM is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution.The density of yellow coloration is read by absorbance at 450 nm and is quantitatively proportional to the amount of sample CKM captured in the well.
ELISA Kit Range1.56 - 100 ng/mL
ELISA Kit Recovery
MatrixRecovery Range (%)Average (%)
Serum (n=5)80-9980
EDTA Plasma (n=5)93-10497
Heparin Plasma (n=5)97-105101
ELISA Kit ReproducibilityIntra-assay Precision: 3 samples with known low, middle and high level CKM was tested with 20 replicates across one plate.
Inter-assay Precision: 3 samples with known low, middle and high level CKM was tested on three different plates with 8 replicates, respectively.
Intra-Assay CV: <10%
Inter-Assay CV: <12%
ELISA Kit Component
Anti-CKM Microplate96 Wells (12 x 8 Well Strips)
CKM Lyophilized Standard2 x 100 ng
100X Biotinylated CKM Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, plasma and other biological fluids
Sensitivity< 0.57 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Creatine Kinase, Muscle (CKM). No significant cross-reactivity or interference between Creatine Kinase, Muscle (CKM) and analogues was observed.

Creatine kinase/a-crystallin interaction functions in cataract development. Biochem Biophys Rep. 22, 100748 (2020). 32154391

Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolCKM
Alias SymbolsCPK-M
NCBI Gene Id100009056
Protein NameCreatine kinase M-type
Description of TargetReversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate). Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa.
Uniprot IDP00563
Protein Accession #NP_001075708.1
Nucleotide Accession #NM_001082239.1
Protein Size (# AA)381
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