CKB ELISA Kit (Mouse) (OKEH06448)

Catalog No: OKEH06448

Size:96 Wells

Mouse CKB ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Mouse
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~3 Hours
• ELISA Kit Principle: Aviva Systems Biology Ckb ELISA Kit (Mouse) (OKEH06448) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Ckb has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Ckb is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Ckb captured in the well.
• ELISA Kit Range: 0.312 - 20ng/mL
• ELISA Kit Reproducibility: Three samples concentrations were measured in replicate within an assay plate and across replicate assays to assess Mean Intra- and Mean Inter-Assay Precision.
  Mean Intra-Assay Precision - < 7.8% (n = 20)
  Mean Inter-Assay Precision - < 10.2% (n = 20)
• ELISA Kit Component:
  • H3F3C Microplate: 96 Wells (12 x 8 Well strips)
  • H3F3C Lyophilized Standard: 2
  • 100X Biotinylated H3F3C Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • Stop Solution: 1 x 10 mL
  • TMB Substrate: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, plasma, tissue homogenates, cell culture supernatants and other biological fluids.
• Sensitivity: 0.189 ng/mL
• Specificity: Natural and recombinant Mouse Creatine kinase B-type
• Publications:

  Creatine kinase/a-crystallin interaction functions in cataract development. Biochem Biophys Rep. 22, 100748 (2020). 32154391

• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: CKB
• Alias Symbols: Bck, Ck3, B-CK, Ck-3, Ckbb, CPK-B
• NCBI Gene Id: 12709
• Protein Name: Creatine kinase B-type
• Description of Target: Reversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate). Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa.
• Uniprot ID: Q04447
• Protein Accession #: NP_660295
• Nucleotide Accession #: NM_145252