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Catalog No: OKEH06448
Size:96T
Price: $625.00
SKU
OKEH06448
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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  • $55: Antibody & Protein in US
  • $55 + $25/Kit in US
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityMouse, Mus musculus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~3 Hours
ELISA Kit PrincipleAviva Systems Biology Ckb ELISA Kit (Mouse) (OKEH06448) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Ckb has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Ckb is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Ckb captured in the well.
ELISA Kit Range0.312-20ng/mL
ELISA Kit ReproducibilityThree samples concentrations were measured in replicate within an assay plate and across replicate assays to assess Mean Intra- and Mean Inter-Assay Precision.
Mean Intra-Assay Precision - < 7.8% (n = 20)
Mean Inter-Assay Precision - < 10.2% (n = 20)
ELISA Kit Component
ComponentAmount
H3F3C Microplate96 Wells (12 x 8 Well strips)
H3F3C Lyophilized Standard2
100X Biotinylated H3F3C Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
Stop Solution1 x 10 mL
TMB Substrate1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, plasma, tissue homogenates, cell culture supernatants and other biological fluids.
Sensitivity0.189 ng/mL
SpecificityNatural and recombinant Mouse Creatine kinase B-type
Publications

Creatine kinase/a-crystallin interaction functions in cataract development. Biochem Biophys Rep. 22, 100748 (2020). 32154391

Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Description
Gene SymbolCkb
Gene Full Namecreatine kinase, brain
Alias SymbolsBck;B-CK;Ck3;Ck-3;Ckbb;CPK-B;creatine kinase B chain;creatine kinase B-type;creatine phosphokinase B-type.
NCBI Gene Id12709
Protein NameQ04447
Description of TargetCreatine kinase B-type
Uniprot IDhttps://www.uniprot.org/uniprot/Q04447
Protein Accession #NP_660295
Nucleotide Accession #NM_145252
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