Catalog No: OKEH08472
Size:96T
Price: $875.00
SKU
OKEH08472
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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  • $55: Antibody & Protein in US
  • $55 + $25/Kit in US
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityCanis lupus, Dog
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit PrincipleAviva Systems Biology CKB ELISA Kit (Dog) (OKEH08472) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CKB has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CKB is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample CKB captured in well.
ELISA Kit Range0.156-10ng/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 91% (n = 20)
ELISA Kit ReproducibilityMean Intra-assay CV%: <=7.1% (n = 20)
Mean Inter-assay CV%: <=10.5% (n = 20)
ELISA Kit Component
ComponentAmount
CKB Microplate96 Wells (12 x 8 Well strips)
CKB Lyophilized Standard2
100X Biotinylated CKB Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Additional InformationFunction: Reversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate). Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa.
::Subcellular Location: Cytoplasm
Reconstitution and StorageStore as indicated in product manual.
Sensitivity0.058ng/mL
SpecificityNatural and recombinant Dog Creatine kinase B-type
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolLOC100855552
Gene Full Namecreatine kinase B-type
Alias SymbolsB-CK;CKB;Creatine kinase B chain;creatine kinase B-type;creatine kinase, brain;Creatine phosphokinase M-type.
NCBI Gene Id100855552
Protein NameP05124
Description of TargetCreatine kinase B-type
Uniprot IDhttps://www.uniprot.org/uniprot/P05124
Protein Accession #XP_003639259.1
Nucleotide Accession #XM_003639211.3
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