CKB Chemi-Luminescent ELISA Kit (Human) (OKCD03376)

Catalog No: OKCD03376

Size:96WELLS

Human CKB ELISA Kit Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Chemiluminescent immunoassay
• ELISA Kit Detection Method: Chemiluminescent
• ELISA Kit Duration: 2h, 40min
• ELISA Kit Principle: The microplate provided in this kit has been pre-coated with an antibody specific to Creatine Kinase B (CK-BB). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Creatine Kinase B (CK-BB). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Creatine Kinase B (CK-BB) level in the sample or standard.
• ELISA Kit Range: 0.27-200ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Creatine Kinase B (CK-BB) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Creatine Kinase B (CK-BB) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • CKB Microplate: 96 Wells (12 x 8 Well strips)
  • CKB Lyophilized Standard: 2
  • 100X CKB Biotinylated Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • 100X Luminol Substrate: 1 x 2 mL
  • Substrate Diluent: 1 x 20 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Tissue homogenates, cell lysates and other biological fluids
• Sensitivity: 0.27 ng/mL
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: CKB
• Gene Full Name: creatine kinase B
• Alias Symbols: BCK;B-CK;brain creatine kinase;CKBB;CPK-B;creatine kinase B chain;creatine kinase brain;creatine kinase brain-type;creatine kinase B-type;creatine phosphokinase B-type;epididymis luminal protein 211;epididymis secretory protein Li 29;HEL-211;HEL-S-29.
• NCBI Gene Id: 1152
• Protein Name: Creatine kinase B-type
• Description of Target: Reversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate). Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa.
• Uniprot ID: P12277
• Protein Accession #: NP_001814
• Nucleotide Accession #: NM_001823