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Catalog No: OKCA01191
Size:96 Wells
Price: $700.00
SKU
OKCA01191
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
COA DatasheetClick Hereto download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHuman
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3.5 Hours
ELISA Kit Linearity
DilutionMean Recovery (%)Range (%)
Neat8683-89
1:29591-99
1:48783-90
1:89992-103
ELISA Kit PrincipleAviva Systems Biology CKAP4 ELISA Kit (Human) (OKCA01191) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CKAP4 has been pre-coated onto a 96-well plate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CKAP4 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample CKAP4 captured in the well.
ELISA Kit Range23.44 - 1,500 pg/mL
ELISA Kit Recovery
SampleMean Recovery (%)Range (%)
Serum (n=5)8683-89
EDTA Plasma (n=4)10095-105
ELISA Kit ReproducibilityIntra-assay Precision: 3 samples with known low, middle and high level OKCA01191 were tested with 20 replicates across one plate.
Inter-assay Precision: 3 samples with known low, middle and high level OKCA01191 were tested on three different plates with 8 replicates, respectively.
Intra-Assay CV: <8%
Inter-Assay CV: <10%
ELISA Kit Component
ComponentAmount
CKAP4 Microplate96 Wells (12 x 8 Well strips)
CKAP4 Lyophilized Standard2 x 1.5 ng
100X Biotinylated CKAP4 Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 50 mL
Detector Antibody Diluent1 x 15 mL
Conjugate Diluent1 x 15 mL
25X Wash Buffer1 x 20 mL
Stop Solution1 x 10 mL
TMB Substrate1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates, Cell Lysates
Sensitivity5.86 pg/mL
Publications

Zhenhua Du, et al. Quantitative proteomics identifies a plasma multi-protein model for detection of hepatocellular carcinoma. 15552 (2020). 32968147

Assay InfoAssay Methodology: Quantitative Sanadwich ELISA
Protocol Information
Description
Gene SymbolCKAP4
Gene Full Namecytoskeleton associated protein 4
Alias Symbolsp63, CLIMP63, CLIMP-63, ERGIC-63
NCBI Gene Id10970
Protein NameCytoskeleton-associated protein 4
Description of TargetHigh-affinity epithelial cell surface receptor for APF. Mediates the anchoring of the endoplasmic reticulum to microtubules.
Uniprot IDQ07065
Protein Accession #NP_006816.2
Nucleotide Accession #NM_006825.3
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