Size:96 Wells
$610.00
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SKU
OKCA01191
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CKAP4 ELISA Kit (Human) (OKCA01191)

Catalog#: OKCA01191
Domestic: within 1-2 weeks delivery | International: 1-2 weeks
More Information
Predicted Species Reactivity Human
Kit Detection Method Colorimetric, OD450 nm
Kit Duration ~ 3.5 Hours
Kit Linearity
DilutionMean Recovery (%)Range (%)
Neat8683-89
1:29591-99
1:48783-90
1:89992-103
Kit Principle Aviva Systems Biology CKAP4 ELISA Kit (Human) (OKCA01191) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CKAP4 has been pre-coated onto a 96-well plate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CKAP4 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample CKAP4 captured in the well.
Kit Range 23.44 - 1,500 pg/mL
Kit Recovery
SampleMean Recovery (%)Range (%)
Serum (n=5)8683-89
EDTA Plasma (n=4)10095-105
Kit Reproducibility Intra-assay Precision: 3 samples with known low, middle and high level OKCA01191 were tested with 20 replicates across one plate.
Inter-assay Precision: 3 samples with known low, middle and high level OKCA01191 were tested on three different plates with 8 replicates, respectively.
Intra-Assay CV: <8%
Inter-Assay CV: <10%
Kit Component
ComponentAmount
CKAP4 Microplate96 Wells (12 x 8 Well strips)
CKAP4 Lyophilized Standard2 x 1.5 ng
100X Biotinylated CKAP4 Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 50 mL
Detector Antibody Diluent1 x 15 mL
Conjugate Diluent1 x 15 mL
25X Wash Buffer1 x 20 mL
Stop Solution1 x 10 mL
TMB Substrate1 x 10 mL
Reconstitution and Storage Store as indicated in product manual.
Sample Type Serum, Plasma, Tissue Homogenates, Cell Lysates
Sensitivity 5.86 pg/mL
Datasheets/Manuals Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Assay Info Assay Methodology: Quantitative Sanadwich ELISA
Gene Symbol CKAP4
Official Gene Full Name cytoskeleton associated protein 4
Alias Symbols CLIMP-63, ERGIC-63, MGC99554, p63, transmembrane protein (63kD), endoplasmic reticulum/Golgi intermediate compartment|type-II transmembrane protein p63
NCBI Gene Id 10970
Protein Name Cytoskeleton-associated protein 4
Description of Target High-affinity epithelial cell surface receptor for APF. Mediates the anchoring of the endoplasmic reticulum to microtubules.
Swissprot Id Q07065
Protein Accession # NP_006816.2
Nucleotide Accession # NM_006825.3
Tissue Tool Find tissues and cell lines supported by DNA array analysis to express CKAP4.
RNA Seq Find tissues and cell lines supported by RNA-seq analysis to express CKAP4.
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