CK8 Polyclonal Antibody (OABB00249)

Catalog No: OABB00249 (Formerly GWB-BBP171)

Size:100UG

Hela Cell Lysate

IHC(P): Human Mammary Cancer Tissue

Product Info

• Tested Species Reactivity: Human
• Predicted Species Reactivity: Hamster|Human|Mouse|Rat
• Product Format: Lyophilized. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
• Clonality: Polyclonal
• Clone: Polyclonal
• Isotype: Rabbit IgG
• Application: Immunohistochemistry|Western blot
• Additional Information: Notes: Tested Species: In-house tested species with positive results.
  Predicted Species: Species predicted to be fit for the product based on sequence similarities.
  By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections.
  Other applications have not been tested.
  Optimal dilutions should be determined by end users.
  
  Background: Keratin, type II cytoskeletal 8, also known as cytokeratin-8 (CK-8) or keratin-8 (K8) is a keratin protein that is encoded in humans by the KRT8 gene. This gene is a member of the type II keratin family clustered on the long arm of chromosome 12. Type I and type II keratins heteropolymerize to form intermediate-sized filaments in the cytoplasm of epithelial cells. The product of this gene typically dimerizes with keratin 18 to form an intermediate filament in simple single-layered epithelial cells. This protein plays a role in maintaining cellular structural integrity and also functions in signal transduction and cellular differentiation. Mutations in this gene cause cryptogenic cirrhosis. Alternatively spliced transcript variants have been found for this gene.
  Background: Cytokeratin 8 (CK8), also known as Keratin 8 (K8), is a type II keratin. Among the various intermediate filament (IF) proteins, cytokeratin 8 (CK8) is especially remarkable as it is produced early in embryogenesis, is the only type-II CK occurring in many simple epithelial cells, and can also be synthesized in certain non-epithelial cells. Cytokeratin 8 mRNA was abundant in fetal small intestine, placenta, pancreas, lung, liver, and kidney. Levels of cytokeratin 8 mRNA in placenta increased slightly during pregnancy. He et al. (2002) presented evidence that K8 is a new cytoplasmic target for JNK in Fas receptor-mediated signaling. The functional significance of this phosphorylation could relate to egulation of JNK signaling and/or regulation of keratin dynamics.
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Immunogen: Polypeptide
• Purification: Affinity Purified
• Predicted Homology Based on Immunogen Sequence: Human; Mouse; Rat
• Concentration: 500 ug/ml
• Specificity: No cross reactivity with other proteins.
• Application Info: Western blot: 0.1-0.5 ug/ml: Human: Mouse, Rat
  Immunohistochemistry (Paraffin-embedded Section): 0.5-1 ug/ml: Human: Mouse, Rat: By Heat
• Reference: 1) Krauss, S.; Franke, W. W. : Organization and sequence of the human gene encoding cytokeratin 8 Gene 86: 241-249, 1990. 2) Yamamoto, R.; Kao, L.-C.; McKnight, C. E.; Strauss, J. F., III : Cloning and sequence of cDNA for
  human placental cytokeratin 8: regulation of the mRNA in trophoblastic cells by cAMP. Molec. Endocr. 4: 370-374, 1990. 3) He, T.; Stepulak, A.; Holmstrom, T. H.; Omary, M. B.; Eriksson, J. E. : The intermediate filament
  protein kinase 8 is a novel cytoplasmic substrate for c-Jun N-terminal kinase. J. Biol. Chem. 277: 10767-10774, 2002.
• Storage Buffer: Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
• Description: Rabbit IgG polyclonal antibody for Keratin, type II cytoskeletal 8(KRT8) detection. Tested with WB, IHC-P in Human;Mouse;Rat.

Target Info

• Gene Symbol: KRT8
• Gene Full Name: keratin 8
• Alias Symbols: CARD2;CK8;CK-8;CYK8;cytokeratin-8;K2C8;K8;keratin 8, type II;keratin, type II cytoskeletal 8;Keratin-8;KO;type-II keratin Kb8.
• NCBI Gene Id: 3856
• Protein Name: Keratin, type II cytoskeletal 8
• Description of Target: Together with KRT19, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle.
• Uniprot ID: P05787
• Molecular Weight: 53704 MW