Catalog No: OKEH08664
Size:96T
Price: $725.00
SKU
OKEH08664
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
Please contact customer service when ordering 10+ kits.
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- Toll Free: 888-880-0001
- Phone: 858-552-6979
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Shipping Info:
- $55: Antibody & Protein in US
- $55 + $25/Kit in US
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Datasheets/Manuals | Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit. |
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Predicted Species Reactivity | Homo sapiens, Human | ||||||||||||||||||||||
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Application | Enzyme-linked Immunosorbent assay-Sandwich | ||||||||||||||||||||||
ELISA Kit Detection Method | Colorimetric, OD450 nm | ||||||||||||||||||||||
ELISA Kit Principle | Aviva Systems Biology CIP2A ELISA Kit (Human) (OKEH08664) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CIP2A has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CIP2A is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample CIP2A captured in well. | ||||||||||||||||||||||
ELISA Kit Range | 78-5000pg/mL | ||||||||||||||||||||||
ELISA Kit Recovery | Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 80% (n = 20) | ||||||||||||||||||||||
ELISA Kit Reproducibility | Mean Intra-assay CV%: <=5.1% (n = 20) Mean Inter-assay CV%: <=8.2% (n = 20) | ||||||||||||||||||||||
ELISA Kit Component |
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Additional Information | Function: Oncoprotein that inhibits PP2A and stabilizes MYC in human malignancies. Promotes anchorage-independent cell growth and tumor formation. | ||||||||||||||||||||||
:: | Subcellular Location: Membrane Single-pass membrane protein Cytoplasm Slightly concentrates in the perinuclear region (PubMed:12118381). | ||||||||||||||||||||||
:: | Unigene: Hs.591308 String: 9606.ENSP00000295746 | ||||||||||||||||||||||
:: | KEGG: hsa:57650 MIM: 610643 | ||||||||||||||||||||||
Reconstitution and Storage | Store as indicated in product manual. | ||||||||||||||||||||||
Sensitivity | 35pg/mL | ||||||||||||||||||||||
Specificity | Natural and recombinant Human Protein CIP2A | ||||||||||||||||||||||
Assay Info | Assay Methodology: Quantitative Sandwich ELISA |
Gene Symbol | CIP2A |
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Gene Full Name | cellular inhibitor of PP2A |
Alias Symbols | cancerous inhibitor of PP2A;cancerous inhibitor of protein phosphatase 2A;cell proliferation regulating inhibitor of protein phosphatase 2A;KIAA1524;NOCIVA;p90;p90 autoantigen;protein CIP2A. |
NCBI Gene Id | 57650 |
Protein Name | Q8TCG1 |
Description of Target | Protein CIP2A |
Uniprot ID | https://www.uniprot.org/uniprot/Q8TCG1 |
Protein Accession # | NP_065941.2 |
Nucleotide Accession # | NM_020890.2 |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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