Catalog No: OKEH08664
Size:96T
Price: $725.00
SKU
OKEH08664
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CIP2A ELISA Kit (Human) (OKEH08664)

Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHomo sapiens, Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit PrincipleAviva Systems Biology CIP2A ELISA Kit (Human) (OKEH08664) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CIP2A has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CIP2A is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample CIP2A captured in well.
ELISA Kit Range78-5000pg/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 80% (n = 20)
ELISA Kit ReproducibilityMean Intra-assay CV%: <=5.1% (n = 20)
Mean Inter-assay CV%: <=8.2% (n = 20)
ELISA Kit Component
ComponentAmount
CIP2A Microplate96 Wells (12 x 8 Well strips)
CIP2A Lyophilized Standard2
100X Biotinylated CIP2A Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Additional InformationFunction: Oncoprotein that inhibits PP2A and stabilizes MYC in human malignancies. Promotes anchorage-independent cell growth and tumor formation.
::Subcellular Location: Membrane Single-pass membrane protein Cytoplasm Slightly concentrates in the perinuclear region (PubMed:12118381).
::Unigene: Hs.591308
String: 9606.ENSP00000295746
::KEGG: hsa:57650
MIM: 610643
Reconstitution and StorageStore as indicated in product manual.
Sensitivity35pg/mL
SpecificityNatural and recombinant Human Protein CIP2A
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolCIP2A
Gene Full Namecellular inhibitor of PP2A
Alias Symbolscancerous inhibitor of PP2A;cancerous inhibitor of protein phosphatase 2A;cell proliferation regulating inhibitor of protein phosphatase 2A;KIAA1524;NOCIVA;p90;p90 autoantigen;protein CIP2A.
NCBI Gene Id57650
Protein NameQ8TCG1
Description of TargetProtein CIP2A
Uniprot IDhttps://www.uniprot.org/uniprot/Q8TCG1
Protein Accession #NP_065941.2
Nucleotide Accession #NM_020890.2
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