CILP ELISA Kit (Mouse) (OKEH03307)

Catalog No: OKEH03307

Size:96T

Mouse CILP ELISA kit standard curve

Product Info

• Predicted Species Reactivity: Mouse, Mus musculus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva Systems Biology CILP ELISA Kit (Mouse) (OKEH03307) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Cilp has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for Cilp is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Cilp captured in well.
• ELISA Kit Range: 78-5000pg/ml
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 104%
• ELISA Kit Reproducibility: Mean Intra-assay CV%: <= 6.8% (n = 20)
  Mean Inter-assay CV%: <= 9.1% (n = 20)
• ELISA Kit Component:
  • Anti-Cilp Microplate: 96 Wells (12 x 8 Well strips)
  • Cilp Lyophilized Standard: 2
  • Sample Diluent: 1 x 20 mL
  • 100X Biotinylated Cilp Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
• Sensitivity: 40.7 pg/mL
• Specificity: Natural and recombinant Mouse Cartilage intermediate layer protein 1
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: Cilp
• Gene Full Name: cartilage intermediate layer protein, nucleotide pyrophosphohydrolase
• Alias Symbols: 9830114D16;C130036G17Rik;cartilage intermediate layer protein 1;CILP-1.
• NCBI Gene Id: 214425
• Protein Name: Q66K08
• Description of Target: Cartilage intermediate layer protein 1
• Uniprot ID: https://www.uniprot.org/uniprot/Q66K08
• Protein Accession #: NP_775561.1
• Nucleotide Accession #: NM_173385.2
• Protein Size (# AA): 1184
• Molecular Weight: 132 kDa