CIDEC Antibody (OAAF02894)

Catalog No: OAAF02894 (Formerly GWB-ASE076)

Size:100 ug

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue, using CIDEC Antibody. The picture on the right is treated with the synthesized peptide.

Immunofluorescence analysis of HeLa cells, using CIDEC Antibody. The picture on the right is treated with the synthesized peptide.

Product Info

• Predicted Species Reactivity: Human|Mouse|Rat
• Product Format: Liquid
• Clonality: Polyclonal
• Host: Rabbit
• Application: Enzyme-linked immunosorbent assay|Immunocytochemistry|Immunofluorescence|Immunohistochemistry|Immunohistochemistry-Paraffin
• Reconstitution and Storage: -20°C
• Immunogen: The antiserum was produced against synthesized peptide derived from human CIDEC.
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Specificity: CIDEC antibody detects endogenous levels of total CIDEC protein.
• Formulation: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Dilution: The optimal working dilution should be determined by the end user.
• Application Info: IHC: 1:50~1:100; IF: 1:100~1:500; ELISA: 1:5000
• Reference: Liang L., Biochem. J. 370:195-203(2003).
  Ota T., Nat. Genet. 36:40-45(2004).
  The MGC Project Team, Genome Res. 14:2121-2127(2004).

Target Info

• Gene Symbol: CIDEC
• Gene Full Name: cell death inducing DFFA like effector c
• Alias Symbols: cell death activator CIDE-3;Cell death-inducing DFFA-like effector protein C;CIDE3;CIDE-3;fat specific protein 27;Fat-specific protein FSP27 homolog;FPLD5;FSP27.
• NCBI Gene Id: 63924
• Protein Name: Cell death activator CIDE-3
• Description of Target: Binds to lipid droplets and regulates their enlargement, thereby restricting lipolysis and favoring storage. At focal contact sites between lipid droplets, promotes directional net neutral lipid transfer from the smaller to larger lipid droplets. The transfer direction may be driven by the internal pressure difference between the contacting lipid droplet pair. Its role in neutral lipid transfer and lipid droplet enlargement is activated by the interaction with PLIN1. May act as a CEBPB coactivator in the white adipose tissue to control the expression of a subset of CEBPB downstream target genes, including SOCS1, SOCS3, TGFB1, TGFBR1, ID2 and XDH. When overexpressed in preadipocytes, induces apoptosis or increases cell susceptibility to apoptosis induced by serum deprivation or TGFB treatment. As mature adipocytes, that express high CIDEC levels, are quite resistant to apoptotic stimuli, the physiological significance of its role in apoptosis is unclear. May play a role in the modulation of the response to osmotic stress by preventing NFAT5 to translocate into the nucleus and activate its target genes expression.
• Uniprot ID: Q96AQ7
• Protein Accession #: NP_001186480.1
• Molecular Weight: 26 kDa