Ciclosporin ELISA Kit (OKEH02549)

Catalog No: OKEH02549

Size:96T

Ciclosporin ELISA Kit (OKEH02549) standard curve.

Product Info

• Predicted Species Reactivity: All Species
• Application: Enzyme-linked Immunosorbent assay-Competitive
• ELISA Kit Detection Method: Colorimetric, OD 450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva Systems Biology Ciclosporin ELISA Kit (OKEH02549) is based on a competitive enzyme immunoassay technique. The microtiter well-plate in this kit has been pre-coated with an anti-Ciclosporin antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated Ciclosporin and incubated. The Ciclosporin found in the sample or standards competes with the biotinylated Ciclosporin for limited binding sites on the immobilized anti-Ciclosporin antibody. Excess unbound biotinylated Ciclosporin and sample or standard Ciclosporin is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated Ciclosporin captured in the well and inversely proportional to the amount of Ciclosporin which was contained in the sample or standard.
• ELISA Kit Range: 3.12-200ng/mL
• ELISA Kit Recovery: Mean recovery when spiking into Serum and Plasma = 96%
• ELISA Kit Reproducibility: Mean Intra-assay CV%: < 4.3% (n = 20)
  Mean Inter-assay CV%: < 6.5% (n = 20)
• ELISA Kit Component:
  • Anti-Ciclosporin Microplate: 96 Wells (12 x 8 Well strips)
  • Ciclosporin Lyophilized Standard: 2 x 200 ng
  • Sample Diluent: 1 x 20 mL
  • 100X Ciclosporin-Biotin Complex: 1 x 60 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Biotin Complex Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Additional Information: Target Abbr: CsA
  Target Name: Ciclosporin
  Pubchem: 5284373
  Chemical formula: C62H111N11O12
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, plasma, tissue homogenates, cell culture supernatants and other biological fluids
• Sensitivity: 1.28 ng/mL
• Specificity: Natural and recombinant General Ciclosporin
• Assay Info: Assay Methodology: Quantitative Competitive ELISA

Target Info

• Alias Symbols: CsA, Ciclosporin, CsA, Cyclosporine A
• Protein Name: Ciclosporin
• Description of Target: Ciclosporin (INN/BAN), cyclosporine (USAN), cyclosporin (former BAN), or cyclosporin A is an immunosuppressant drug widely used in organ transplantation to prevent rejection. It reduces the activity of the immune system by interfering with the activity of T cells.