CIB1 Antibody (OAAD00241)

Catalog No: OAAD00241

Size:0.1ml

western blot analysis using CIB1 mouse mAb against truncated CIB1 recombinant protein (1) and A431 cell lysate (2).

Immunohistochemical analysis of paraffinembedded human thalamus (left) and glioma (right) tissue, showing membrane localization using CIB1 mouse mAb with DAB staining.

Product Info

• Predicted Species Reactivity: Human
• Clonality: Monoclonal
• Clone: 5A1F5E12, 5A1H7E12
• Host: Mouse
• Application: WB, IHC
• Reconstitution and Storage: Store at 4C, for long term storage, store at -20C.
• Immunogen: Purified recombinant fragment of CIB1 expressed in E. Coli.
• Formulation: Ascitic fluid containing 0.03% sodium azide.
• Application Info: Western Bloting: 1/500 - 1/2000.
  Immunohistochemistry: 1/200 - 1/1000.
  ELISA: Propose dilution 1/10000.
  Not yet tested in other applications.
  Determining optimal working dilutions by titration test.
• Reference: 1. Holly R. Gentry, Alex U. Singer, Laurie Betts. J. Biol. Chem., Mar 2005; 280: 8407 - 8415.
  2. Carl White, Jun Yang, Mervyn J. Monteiro. J. Biol. Chem., Jul 2006; 281: 20825 ¨C 20833.

Target Info

• Alias Symbols: CIB, CIBP, KIP1, PRKDCIP, SIP2-28
• NCBI Gene Id: 10519
• Description of Target: CIB1(also designated calcium and integrin binding 1 or calmyrin), with 191-amino acid protein(about 21kDa), belongs to the calcium-binding protein family.CIB1 is known to interact with DNA-dependent protein kinase and may play a role in kinase-phosphatase regulation of DNA end joining.CIB1 is an EF-hand-containing protein that binds multiple effector proteins, including the platelet alpha(IIb)beta(3) integrin and several serine/threonine kinases and potentially modulates their function.CIB1 regulates platelet aggregation in hemostasis through a specific interaction with the alpha(IIb) cytoplasmic domain of platelet integrin alpha(IIb)beta(3). CIB1 is also ubiquitously expressed activating and inhibiting protein ligand of the InsP3R.
• Uniprot ID: Q99828