CHTF18 Antibody - C-terminal region (OAAB09272)

Catalog No: OAAB09272

Size:400ul

CHTF18 Antibody (C-term) (Cat. #OAAB09272) western blot analysis in NCI-H292 cell line lysates (35ug/lane).This demonstrates the CHTF18 antibody detected the CHTF18 protein (arrow).

Product Info

• Predicted Species Reactivity: Human
• Product Format: Liquid. PBS with 0.09% (W/V) sodium azide.
• Clonality: Polyclonal
• Isotype: Ig
• Host: Rabbit
• Application: WB
• Additional Information: Function: Chromosome cohesion factor involved in sister chromatid cohesion and fidelity of chromosome transmission. Component of one of the cell nuclear antigen loader complexes, CTF18-replication factor C (CTF18-RFC), which consists of CTF18, CTF8, DCC1, RFC2, RFC3, RFC4 and RFC5. The CTF18-RFC complex binds to single- stranded and primed DNAs and has weak ATPase activity that is stimulated by the presence of primed DNA, replication protein A (RPA) and by proliferating cell nuclear antigen (PCNA). The CTF18- RFC complex catalyzes the ATP-dependent loading of PCNA onto primed and gapped DNA. It also interacts with and stimulates DNA polymerase POLH
  Cellular Location: Nucleus. Note=Associates with chromatin during S phase
  
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: This CHTF18 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 914-943 amino acids from the C-terminal region of human CHTF18.
• Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.
• Predicted Homology Based on Immunogen Sequence: Human
• Concentration: Approximately 0.5mg/ml. Actual concentration varies with each lot.
• Intended Use: CHTF18 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
• Application Info: WB: 1:1000

Target Info

• Gene Symbol: CHTF18
• Alias Symbols: CHL12, Ctf18, RUVBL, C16orf41, C321D2.2, C321D2.3, C321D2.4
• NCBI Gene Id: 63922
• Protein Name: Chromosome transmission fidelity protein 18 homolog
• Description of Target: CHTF18, CHTF8 (MIM 613202), and DCC1 (DSCC1; MIM 613203) are components of an alternative replication factor C (RFC) (see MIM 600404) complex that loads PCNA (MIM 176740) onto DNA during S phase of the cell cycle (Merkle et al., 2003 [PubMed 12766176]; Bermudez et al., 2003 [PubMed 12930902]).
• Uniprot ID: Q8WVB6
• Protein Accession #: NP_071375.1
• Molecular Weight: 107383 Da