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Catalog No: OCOA18568
Size:20UG
Price: $169.00
SKU
OCOA18568
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CHST8 Protein Lysate (OCOA18568)

Datasheets/ManualsPrintable datasheet for CHST8 Protein Lysate (OCOA18568)
Product Info
Predicted Species ReactivityHomo sapiens
Product FormatLyophilized
HostHEK293T
Additional InformationComponents: 1 vial of 20 ug lyophilized gene specific transient over-expression cell lysate
::Preparation: HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins.
Reconstitution and StorageThe lysate can be shipped at ambient temperature. Upon receiving, store the sample at -20C. Lysate samples are stable for 12 months from date of receipt when stored at -20C. Avoid repeated freeze-thaw cycles after reconstitution. Lysate samples can be reconstituted with SDS Sample Buffer. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes
TagC-MYC/DDK
DescriptionCHST8 HEK293T cell transient overexpression lysate (as WB positive control)
Gene SymbolCHST8
Gene Full Namecarbohydrate sulfotransferase 8
Alias Symbolscarbohydrate (N-acetylgalactosamine 4-0) sulfotransferase 8;carbohydrate sulfotransferase 8;GalNAc-4-O-sulfotransferase 1;GalNAc4ST;galNAc4ST-1;GALNAC4ST1;GALNAC-4-ST1;N-acetylgalactosamine-4-O-sulfotransferase 1;PSS3.
NCBI Gene Id64377
Protein NameNM_001127896, NP_001121368, Q9H2A9
Description of TargetCarbohydrate sulfotransferase 8, carbohydrate sulfotransferase 8 [Homo sapiens], Homo sapiens carbohydrate sulfotransferase 8 (CHST8), transcript variant 1, mRNA
Uniprot IDhttps://www.uniprot.org/uniprot/Q9H2A9
Protein Accession #https://www.ncbi.nlm.nih.gov/protein/NP_001121368
Nucleotide Accession #https://www.ncbi.nlm.nih.gov/nuccore/NM_001127896
Molecular Weight48.7 kDa
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