Catalog No: OKCD01999 (Formerly GWB-KBBYY9)
Size:96 Wells
Price: $675.00
SKU
OKCD01999
Availability: Domestic: within 2-3 weeks delivery | International: 2-3 weeks
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHuman
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~3.5 Hours
ELISA Kit Linearity
Sample1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
Serum (n=5)82-104%79-91%91-98%79-102%
ELISA Kit PrincipleAviva Systems Biology CHST7 ELISA Kit (Human) (OKCD01999) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CHST7 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CHST7 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution.The density of yellow coloration is read by absorbance at 450 nm and is quantitatively proportional to the amount of sample CHST7 captured in the well.
ELISA Kit Range78.1 - 5,000 pg/mL
ELISA Kit Recovery
MatrixRecovery Range (%)Average (%)
Serum89-10397
ELISA Kit ReproducibilityIntra-assay Precision: 3 samples with known low, middle and high level CHST7 was tested with 20 replicates across one plate.
Inter-assay Precision: 3 samples with known low, middle and high level CHST7 was tested on three different plates with 8 replicates, respectively.
Intra-Assay CV: <10%
Inter-Assay CV: <12%
ELISA Kit Component
ComponentAmount
Anti-CHST7 Microplate96 Wells (12 x 8 Well Strips)
CHST7 Lyophilized Standard2 x 5 ng
100X Biotinylated CHST7 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeTissue homogenates, cell lysates and other biological fluids
Sensitivity< 31 pg/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Carbohydrate Sulfotransferase 7 (CHST7). No significant cross-reactivity or interference between Carbohydrate Sulfotransferase 7 (CHST7) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolCHST7
Gene Full NameCarbohydrate sulfotransferase 7
Alias SymbolsGST-5, C6ST-2
NCBI Gene Id56548
Protein NameCarbohydrate sulfotransferase 7
Description of TargetSulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) as sulfonate donor to catalyze the transfer of sulfate to position 6 of non-reducing N-acetylglucosamine (GlcNAc) residues. Preferentially acts on mannose-linked GlcNAc. Also able to catalyze the transfer of sulfate to position 6 of the N-acetylgalactosamine (GalNAc) residue of chondroitin. Also acts on core 2 mucin-type oligosaccharide and N-acetyllactosamine oligomer with a lower efficiency. Has weak or no activity toward keratan sulfate and oligosaccharides containing the Galbeta1-4GlcNAc. Catalyzes 6-O-sulfation of beta-benzyl GlcNAc but not alpha- or beta-benzyl GalNAc.
Swissprot IdQ9NS84
Protein Accession #NP_063939.2
Nucleotide Accession #NM_019886.3
Protein Size (# AA)486
Tissue ToolFind tissues and cell lines supported by DNA array analysis to express CHST7.
RNA SeqFind tissues and cell lines supported by RNA-seq analysis to express CHST7.
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