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Catalog No: OKCD00895 (Formerly GWB-KBBRH2)
Size:96WELLS
Price: $675.00
SKU
OKCD00895
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Datasheets/ManualsPrintable datasheet for CHRNB3 ELISA Kit (Human) (OKCD00895)
Product Info
Predicted Species ReactivityHomo sapiens|Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cholinergic Receptor, Nicotinic, Beta 3 (CHRNb3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cholinergic Receptor, Nicotinic, Beta 3 (CHRNb3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cholinergic Receptor, Nicotinic, Beta 3 (CHRNb3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Cholinergic Receptor, Nicotinic, Beta 3 (CHRNb3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.312-20ng/mL
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Cholinergic Receptor, Nicotinic, Beta 3 (CHRNb3) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Cholinergic Receptor, Nicotinic, Beta 3 (CHRNb3) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-CHRNB3 Microplate96 Wells (12 x 8 Well Strips)
CHRNB3 Lyophilized Standard2 x 20 ng
100X Biotinylated CHRNB3 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeTissue homogenates and other biological fluids.
Sensitivity< 0.125 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Cholinergic Receptor, Nicotinic, Beta 3 (CHRNb3). No significant cross-reactivity or interference between Cholinergic Receptor, Nicotinic, Beta 3 (CHRNb3) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolCHRNB3
Gene Full Namecholinergic receptor nicotinic beta 3 subunit
Alias Symbolsacetylcholine receptor, neuronal nicotinic, beta-3 subunit;acetylcholine receptor, nicotinic, beta 3 (neuronal);cholinergic receptor, nicotinic beta 3;cholinergic receptor, nicotinic, beta 3 (neuronal);cholinergic receptor, nicotinic, beta polypeptide 3;neuronal acetylcholine receptor subunit beta-3.
NCBI Gene Id1142
Protein NameNeuronal acetylcholine receptor subunit beta-3
Description of TargetAfter binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane.
Uniprot IDQ05901
Protein Accession #NP_000740
Nucleotide Accession #NM_000749.3
Protein Size (# AA)458
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