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Catalog No: OKCD03375
Size:96WELLS
Price: $800.00
SKU
OKCD03375
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CHRNA1 Chemi-Luminescent ELISA Kit (Human) (OKCD03375)

Datasheets/ManualsPrintable datasheet for CHRNA1 Chemi-Luminescent ELISA Kit (Human) (OKCD03375)
Product Info
Predicted Species ReactivityHomo sapiens|Human
ApplicationChemiluminescent immunoassay
ELISA Kit Detection MethodChemiluminescent
ELISA Kit Duration2h, 40min
ELISA Kit PrincipleThe microplate provided in this kit has been pre-coated with an antibody specific to Cholinergic Receptor, Nicotinic, Alpha 1 (CHRNa1). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Cholinergic Receptor, Nicotinic, Alpha 1 (CHRNa1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Cholinergic Receptor, Nicotinic, Alpha 1 (CHRNa1) level in the sample or standard.
ELISA Kit Range68.6-50,000pg/mL
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Cholinergic Receptor, Nicotinic, Alpha 1 (CHRNa1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Cholinergic Receptor, Nicotinic, Alpha 1 (CHRNa1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
CHRNA1 Microplate96 Wells (12 x 8 Well strips)
CHRNA1 Lyophilized Standard2
100X CHRNA1 Biotinylated Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
100X Luminol Substrate1 x 2 mL
Substrate Diluent1 x 20 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeTissue homogenates and other biological fluids
Sensitivity0.78 ng/mL
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolCHRNA1
Gene Full Namecholinergic receptor nicotinic alpha 1 subunit
Alias Symbolsacetylcholine receptor subunit alpha;acetylcholine receptor, nicotinic, alpha 1 (muscle);ACHRA;ACHRD;cholinergic receptor, nicotinic alpha 1;cholinergic receptor, nicotinic, alpha 1 (muscle);cholinergic receptor, nicotinic, alpha polypeptide 1 (muscle);CHRNA;CMS1A;CMS1B;CMS2A;FCCMS;muscle nicotinic acetylcholine receptor;nicotinic acetylcholine receptor alpha subunit;nicotinic cholinergic receptor alpha 1;SCCMS.
NCBI Gene Id1134
Protein NameAcetylcholine receptor subunit alpha
Description of TargetAfter binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane.
Uniprot IDP02708
Protein Accession #NP_000070
Nucleotide Accession #NM_000079
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