Catalog No: OKCD08818
Size:96 Wells
Price: $825.00
Availability: Domestic: within 2-3 weeks delivery | International: 2-3 weeks
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityMouse
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology CHRDL2 ELISA Kit (Mouse) (OKCD08818) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CHRDL2 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CHRDL2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample CHRDL2 captured in the well.
ELISA Kit Range0.156-10ng/mL
ELISA Kit ReproducibilityIntra-assay reproducibility was evaluated using 20 replicates of 3 samples representing low, middle and high level CHRDL2. Inter-assay reproducibility was evaluated with 3 samples representing low, middle and high level CHRDL2 using 8 replicates on each of 3 plates.
Intra-Assay CV <10%
Inter-Assay CV <12%
ELISA Kit Component
CHRDL2 Microplate96 Wells (12 x 8 Well strips)
CHRDL2 Lyophilized Standard2
100X CHRDL2 HRP-Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 2 mL
Stop Solution1 x 20 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeTissue homogenates, cell lysates and other biological fluids
Sensitivity< 0.060ng/mL
Gene SymbolCHRDL2
Gene Full Namechordin-like 2
Alias SymbolsChl, Chl2, 1810022C01Rik
NCBI Gene Id69121
Protein Namechordin-like protein 2
Description of TargetImplicated in tumor angiogenesis (by similarity). may inhibits bmps activity by blocking their interaction with their receptors. has a negative regulator effect on the cartilage formation/regeneration from immature mesenchymal cells, by preventing or reducing the rate of matrix accumulation. may play a role during myoblast and osteoblast differentiation, and maturation.
Swissprot IdQ8VEA6
Protein Accession #NP_001278249.1
Nucleotide Accession #NM_001291320.1
Tissue ToolFind tissues and cell lines supported by RNA-seq analysis to express CHRDL2.
RNA SeqFind tissues and cell lines supported by DNA array analysis to express CHRDL2.
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