CHML Antibody - C-terminal region (OAAB18114)

Catalog No: OAAB18114

Size:400ul

Western blot analysis of lysate from HT-1080 cell line, using CHML Antibody (C-term) (Cat. #OAAB18114).
OAAB18114 was diluted at 1:1000.
A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody.
Lysate at 35ug.
Dilution: 1:1000

Product Info

• Predicted Species Reactivity: Human
• Product Format: Liquid. Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Host: Rabbit
• Application: WB
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: This CHML antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 624-656 amino acids from the C-terminal region of human CHML.
• Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.
• Application Info: IHC-P: 1:25
  FC: 1:25
  WB: 1:1000
• Reference: Cremers F.P.M., et al.Hum. Mol. Genet. 1:71-75(1992).
  Kasper G., et al.Gene 295:27-32(2002).
  Ota T., et al.Nat. Genet. 36:40-45(2004).
  Gregory S.G., et al.Nature 441:315-321(2006).
  Mural R.J., et al.Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.

Target Info

• Gene Symbol: CHML
• Alias Symbols: REP2
• NCBI Gene Id: 1122
• Description of Target: Substrate-binding subunit (component A) of the Rab geranylgeranyltransferase (GGTase) complex. Binds unprenylated Rab proteins and presents the substrate peptide to the catalytic component B. The component A is thought to be regenerated by transferring its prenylated Rab back to the donor membrane. Less effective than CHM in supporting prenylation of Rab3 family.
• Uniprot ID: P26374
• Molecular Weight: 74 kDa