Chloramphenicol ELISA Kit (OKAO00130)

Catalog No: OKAO00130

Size:96 Wells

General Chloramphenicol ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: General
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~4 Hours
• ELISA Kit Principle: Aviva Systems Biology Chloramphenicol ELISA Kit (OKAO00130) is based on standard competitive inhibition enzyme-linked immuno-sorbent assay technology. Chloramphenicol has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells along with a rabbit anti-Chloramphenicol antibody and an anti-rabbit / HRP Conjugated antibody, then incubated. Wells are washed and detection substrates are added and incubated. An enzymatic reaction is produced through the addition of TMB which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm which is quantitatively proportional to the amount of detector antibody bound in the well and inversely proportional to the amount of Sulfadimethoxine in the sample.
• ELISA Kit Range: 0.05-4.05 ppb
• ELISA Kit Recovery:

  Sample	Amount
  Tissue, Liver	85% +/- 20%
  Honey, Intestine, Egg	85% +/- 25%
  Milk, Milk powder, Feed	75% +/- 25%
  Urine, Serum, Plasma	70% +/- 20%

• ELISA Kit Reproducibility: Mean Intra-Assay: CV<8% (n= 3 x 20)
  Mean Inter-Assay: CV<12% (n= 3 x 40)
• ELISA Kit Component:
  • Chloramphenicol Microplate: 96 Wells (12 x 8 Well Strips)
  • Chloramphenicol Standards: 6 x 1 mL
  • Chloramphenicol Rabbit Detector Antibody: 1 x 6 mL
  • Anti-Rabbit / HRP Conjugate: 1 x 11 mL
  • Detection Reagent A: 1 x 6 mL
  • Detection Reagent B: 1 x 6 mL
  • Stop Solution: 1 x 6 mL
  • 20X Wash Buffer: 2 x 20 mL
  • 2X Sample Prep Buffer: 2 x 20 mL
• Additional Information: CAS: 56-75-7
  PubChem: 5959
  Molecular Formula: C11H12Cl2N2O5
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Tissue (fish, shrimp), liver (Chicken, Pork), serum, plasma, urine, honey, intestine, milk, milk powder, egg, feed.
• Sensitivity:

  Sample	Amount
  Tissue, Liver, Honey, Milk	0.025 ppb
  Egg	0.1 ppb
  Urine, Serum, Plasma	0.05 ppb
  Intestine, Feed, Milk powder	0.05 ppb

• Specificity:

  Substance	Cross Reactivity Rate
  Chloramphenicol	100%
  Thiamphenicol	<0.1%
  Florfenicol	<0.1%

• Assay Info: Assay Methodology: Competitive Inhibition ELISA

Target Info

• Alias Symbols: CAP, Chloramphenicol, Chloromycetin, Chlornitromycin, Levomycetin, Chlorocid, Globenicol
• Protein Name: Chloramphenicol
• Description of Target: Chloramphenicol is a semisynthetic, broad-spectrum antibiotic derived from Streptomyces venequelae with primarily bacteriostatic activity. Chloramphenicol diffuses through the bacterial cell wall and reversibly binds to the bacterial 50S ribosomal subunit. The binding interferes with peptidyl transferase activity, thereby prevents transfer of amino acids to the growing peptide chains and blocks peptide bond formation. As a result bacterial protein synthesis is blocked and impede bacterial cell proliferation.
• Molecular Weight: 323.12938 g/mol