CHIMAERIC Human IgE ANTI NP Antibody (OASA00004)

Catalog No: OASA00004 (Formerly GWB-DF0FC3)

Size:2ML

Product Info

• Predicted Species Reactivity: Human
• Product Format: Liquid. None present
• Clonality: Monoclonal
• Clone: JW8/1
• Isotype: IgE
• Host: HUMAN
• Application: Enzyme-linked immunosorbent assay|Functional assay
• Additional Information: Fusion Partners: Plasmids containing chimaeric heavy chain gene were fused with cells of the J558L mouse myeloma cell line.
• Reconstitution and Storage: -20°C
• Immunogen: Hapten, 4-hydroxy-3-nitrophenylacetyl (NP).
• Purification: Tissue Culture Supernatant containing 0.2M Tris/HCl pH7.4 and 5-10% foetal calf serum
• Predicted Homology Based on Immunogen Sequence: Human
• Specificity: The immunoglobulin heavy chain has been produced by the linking of the antigen-binding, variable region genes of a mouse hybridoma to human constant region genes by in vitro DNA recombination procedures. The resulting chimeric antibody is subsequently expressed by the myeloma cell-line J558L after transfection. (The J558L cell-line self secretes a lambda light chain but no heavy chain). Thus a chimeric human IgE antibody specific for NP has been produced.
• Application Info: ELISA: 1/20 - 1/100
  FN: This product contains sodium azide, removal by dialysis is recommended prior to use in functional assays.
• Protocol Information: Citation: 1: Paul-Eugene N, Kolb JP, Sarfati M, Arock M, Ouaaz F, Debre P, Mossalayi DM,Dugas B. Ligation of CD23 activates soluble guanylate cyclase in human monocytes via an L-arginine-dependent mechanism. J Leukoc Biol. 1995 Jan;57(1):160-7.PubMed PMID: 7829968.
  Species: Human
  Experiment Name: cGMP measurements
  Experiment Background: L-NMMA inhibits the IgE-driven cGMP accumulation in IL-4-activated inonocytes.
  Experimental Steps: 1. Monocytes, cultured for 48 h with IL-4 (100 U/mI), were washed. 2. Resuspended at 7 x 106 cells/mI in RPMI 1640 medium containing 200 μM IBMX and without or with L-NMMA (1 mM) for a 90-min preincubation at 37C.3. The cells were then stimulated for 15 min with either control medium, chimeric lgE-NP (20 μ/mI) + NIP-BSA (20 μg/mI), chimeric IgE-NP (20 μg/mI) + anti-IgE (20 μg/mI), or SNP (500 μM). 4. Perchloric extracts were analyzed for cGMP content with a radioimmunoassay kit (NEN).
  Other Reagents Used: KOH, perchloric acid
  Number Of Protocols: 1
• Reference: 1. Neuberger, M. S. et al. (1984) Recombinant antibodies possessing novel effector functions. Nature 312: 604-608.
  2. Neuberger, M. S. et al. (1985) Making novel antibodies by expressing transfected immunoglobulin genes. TIBS 347-349.
  3. Neuberger, M. S. et al. (1985) A hapten-specific chimaeric IgE antibody with Human physiological effector function. Naure 314: 268-270.
  4. Neuberger, M. S. et al. (1986) Construction of novel antibodies by use of DNA transfection: design of plasmid vectors. Phil. Trans. R. Soc. Lond. A317: 425-432.
  5. Sayers, I. et al. (2004) The importance of Lys-352 of human immunoglobulin E in FcepsilonRII/CD23 recognition. J Biol Chem. 279: 35320-5.
  

Target Info

• Protein Name: Ig epsilon chain C region
• Description of Target: CHIMAERIC HUMAN IgE ANTI NP
  
• Uniprot ID: P01854