Catalog No: OKEH02997
Size:96T
Price: $425.00
SKU
OKEH02997
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityMouse, Mus musculus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology CHI3L3 ELISA Kit (Mouse) (OKEH02997) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Chi3l3 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and removed. A biotinylated detector antibody specific for Chi3l3 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Chi3l3 captured in well.
ELISA Kit Range78-5000pg/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 95%
ELISA Kit ReproducibilityMean Intra-assay CV%: <= 6.8% (n = 20)
Mean Inter-assay CV%: <= 9.3% (n = 20)
ELISA Kit Component
ComponentAmount
Anti-Chi3l3 Microplate96 Wells (12 x 8 Well strips)
Chi3l3 Lyophilized Standard2
100X Biotinylated Chi3l3 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
Sensitivity40.8 pg/mL
SpecificityNatural and recombinant Mouse Chitinase-3-like protein 3
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolChil3
Gene Full Namechitinase-like 3
Alias SymbolsAI505981;beta-N-acetylhexosaminidase Ym1;Chi3;Chi3l3;Chitinase-3-like protein 3;chitinase-like protein 3;ECF-L;eosinophil chemotactic cytokine;eosinophil chemotactic factor-L;secreted protein Ym1;Ym1.
NCBI Gene Id12655
Protein NameO35744
Description of TargetChitinase-like protein 3
Uniprot IDhttps://www.uniprot.org/uniprot/O35744
Protein Accession #NP_034022.2
Nucleotide Accession #NM_009892.3
Protein Size (# AA)398
Molecular Weight44 kDa
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