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CHI3L1 ELISA Kit (Pig) (OKCA02487)

96 Wells
$675.00
In Stock
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Gene Symbol:
CHI3L1
NCBI Gene Id:
396865
Alias Symbols:
ASRT7, DKFZp686N19119, FLJ38139, GP39, HC-gp39, HCGP-3P, YKL40, YYL-40, chitinase 3-like 1
Description of Target:
Carbohydrate-binding lectin with a preference for chitin. Has no chitinase activity. May play a role in tissue remodeling and in the capacity of cells to respond to and cope with changes in their environment. Plays a role in T-helper cell type 2 (Th2) inflammatory response and IL-13-induced inflammation, regulating allergen sensitization, inflammatory cell apoptosis, dendritic cell accumulation and M2 macrophage differentiation. Facilitates invasion of pathogenic enteric bacteria into colonic mucosa and lymphoid organs. Mediates activation of AKT1 signaling pathway and subsequent IL8 production in colonic epithelial cells. Regulates antibacterial responses in lung by contributing to macrophage bacterial killing, controlling bacterial dissemination and augmenting host tolerance. Also regulates hyperoxia-induced injury, inflammation and epithelial apoptosis in lung. Stimulates migration and adhesion of cultured vascular smooth muscle cells.
Protein Name:
Chitinase-3-like protein 1
Swissprot Id:
Q29411
Protein Accession #:
NP_001001540
Nucleotide Accession #:
NM_001001540
Predicted Species Reactivity:
Pig
Sample Type:
Serum, Plasma, Tissue Homogenates
Sensitivity:
3.12 ng/mL
Kit Range:
12.5 - 800 ng/mL
Kit Reproducibility:
Mean Intra-Assay: CV <12% (n=20)
Mean Inter-Assay: CV <15% (n=24)
Kit Duration:
~ 3.5 Hours
Kit Principle:
Aviva Systems Biology CHI3L1 ELISA Kit (Pig) (OKCA02487) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CHI3L1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CHI3L1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample or standard CHI3L1 captured in the well.
Kit Component:
ComponentAmount
CHI3L1 Microplate96 Wells (12 x 8 Well strips)
CHI3L1 Lyophilized Standard2
100X Biotinylated CHI3L1 Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 20 mL
Detector Antibody Diluent1 x 10 mL
Conjugate Diluent1 x 10 mL
25X Wash Buffer1 x 20 mL
Stop Solution1 x 10 mL
TMB Substrate1 x 10 mL
Kit Linearity:
DilutionMean Recovery (%)Range (%)
Neat8782-92
1:29690-102
1:48984-95
1:89187-98
Kit Recovery:
Sample TypeMean Recovery (%)Range (%)
Serum (n=5)10095-104
EDTA Plasma (n=4)9288-97
Kit Detection Method:
Colorimetric, OD450 nm
Tissue Tool:
Find tissues and cell lines supported by DNA array analysis to express CHI3L1.
RNA Seq:
Find tissues and cell lines supported by RNA-seq analysis to express CHI3L1.
Datasheets/Manuals:
Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Reconstitution and Storage:
Store as indicated in product manual.
Assay Info:
Assay Methodology: Quantitative Sandwich Immunoassay

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