Size:96 Wells
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CHGA ELISA Kit (Rat) (OKEH03318)

Catalog#: OKEH03318
Domestic: within 1-2 weeks delivery | International: 1-2 weeks
More Information
Predicted Species Reactivity Rat
Kit Detection Method Colorimetric, OD450 nm
Kit Duration ~ 3 Hours
Kit Principle Aviva Systems Biology CHGA ELISA Kit (Rat) (OKEH03318) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Chga has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for Chga is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Chga captured in well.
Kit Range 0.156 - 10ng/mL
Kit Component
Anti-Chga Microplate96 Wells (12 x 8 Well strips)
Chga Lyophilized Standard2
Sample Diluent1 x 20 mL
100X Biotinylated Chga Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and Storage Store as indicated in product manual.
Sample Type Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Sensitivity 0.091 ng/mL
Datasheets/Manuals Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Specificity Natural and recombinant Rat Chromogranin-A
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Gene Symbol CHGA
Official Gene Full Name chromogranin A
Alias Symbols CgA, Chromogranin-A
NCBI Gene Id 1113
Protein Name Chromogranin-A(CgA)
Description of Target Pancreastatin: Strongly inhibits glucose induced insulin release from the pancreas. Catestatin inhibits catecholamine release from chromaffin cells and noradrenergic neurons by acting as a non-competitive nicotinic cholinergic antagonist. Can induce mast cell migration, degranulation and production of cytokines and chemokines.
Swissprot Id P10354
Protein Accession # NP_001266.1
Nucleotide Accession # NM_001275.3
Protein Size (# AA) 457
Molecular Weight 51 kDa
Tissue Tool Find tissues and cell lines supported by DNA array analysis to express Chga.
RNA Seq Find tissues and cell lines supported by RNA-seq analysis to express Chga.
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