Catalog No: OKBB00665
Size:96 Wells
Price: $583.00
SKU
OKBB00665
Availability: Domestic: within 1-2 week delivery | International: within 1-2 week delivery
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHuman
ApplicationELISA
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration2.5 Hours
ELISA Kit PrincipleAviva's human Chemerin ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A polyclonal antibody from goat specific for Chemerin has been precoated onto 96-well plates. Standards(E.coli, V17-S163) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Chemerin is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the human Chemerin amount of sample captured in plate.
ELISA Kit Range78 pg/ml - 5,000 pg/ml
ELISA Kit Reproducibility
Intra-Assay PrecisionInter-Assay Precision
Sample123123
n 161616242424
Mean (pg/mL)4.81526.45.316.728.3
SD0.2690.691.030.4241.2861.896
CV%5.64.63.987.76.7
ELISA Kit Component
ComponentAmount
96-Well plate Pre-coated with Anti-Human Chemerin Antibody1 x (12 x 8 Well Strip)
Lyophilized Recombinant Human Chemerin Standard2 x 50 ng/tube
100X Biotinylated Anti-Human Chemerin Antibody1 x 130 uL
100X Avidin-Biotin-Peroxidase Complex (ABC)1 x 130 uL
Sample Diluent Buffer1 x 30 mL
Antibody Diluent Buffer1 x 12 mL
ABC Diluent Buffer1 x 12 mL
TMB Color Developing Agent1 x 10 mL
TMB Stop Solution1 x 10 mL
10X Wash Buffer1 x 30 mL
Reconstitution and StorageStore at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Sample TypeCell culture supernatants, serum and plasma (heparin, EDTA)
Sensitivity<20 pg/ml
SpecificityNatural and recombinant human Chemerin
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
ImmunogenExpression system for standard: E.coli; Immunogen sequence: V17-S163
DescriptionFor quantitative detection of human Chemerin in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
Gene SymbolRARRES2
Gene Full Nameretinoic acid receptor responder (tazarotene induced) 2
Alias Symbolschemerin, HP10433, RAR-responsive protein TIG2, RARRES2, retinoic acid receptor responder (tazarotene induced) 2, retinoic acid receptor responder protein 2, tazarotene-induced gene 2 protein, TIG2
NCBI Gene Id5919
Protein NameRetinoic acid receptor responder protein 2
Description of TargetChemerin, also known as RARRES2 or TIG2, is a protein that in humans is encoded by the RARRES2 gene. It is mapped to 7q36.1. Chemerin is a potent chemoattractant specific for antigen-presenting cells that requires proteolytic activation and acts as a ligand for the G protein-coupled receptor CMKLR1 (also known as ChemR23). It is a 14 kDa protein secreted in an inactive form as prochemerin and is activated through cleavage of the C-terminus by inflammatory and coagulation serine proteases. Chemerin was found to stimulate chemotaxis of dendritic cells and macrophages to the site of inflammation. What’s more, the active protein has several roles, including that as an adipokine, and is truncated on both termini from the proprotein.
Uniprot IDQ99969
Protein Accession #NP_002880.1
Nucleotide Accession #NM_002889.3
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