CHEK2 Polyclonal Antibody (OABB00608)

Catalog No: OABB00608 (Formerly GWB-BBP530)

Size:100ug

Lane1: MCF-7 Cell Lysate
Lane2: COLO320 Cell Lysate
Lane3: Jurkat Cell Lysate

Product Info

• Tested Species Reactivity: Human, Rat
• Predicted Species Reactivity: Mouse
• Product Format: Lyophilized
• Clonality: Polyclonal
• Clone: Polyclonal
• Isotype: Rabbit IgG
• Application: WB
• Additional Information: Notes: Tested Species: In-house tested species with positive results.
  Predicted Species: Species predicted to be fit for the product based on sequence similarities.
  Other applications have not been tested.
  Optimal dilutions should be determined by end users.
  
  Background: CHK2, a protein kinase that is activated in response to DNA damage, is involved in cell cycle arrest. Mapped on 22q12.1, CHK2 has a potential regulatory region rich in SQ and TQ amino acid pairs. It regulates BRCA1 function after DNA damage by phosphorylating serine-988 of BRCA1. Additionally, CHK2 can be modified by phosphorylation and activated in response to ionizing radiation, and can be also modified in response to hydroxyurea treatment. Furthermore, oligomerization of CHEK2 increases the efficiency of transautophosphorylation, resulting in the release of active CHEK2 monomers that proceed to enforce checkpoint control in irradiated cells. Moreover, CHK2 is a tumor suppressor gene conferring predisposition to sarcoma, breast cancer, and brain tumors, and that their observations provided a link between the central role of p53 inactivation in human cancer and the well-defined G2 checkpoint in yeast. There is a wide expression of small amounts of CHK2 mRNA with larger amounts in human testis, spleen, colon, and peripheral blood leukocytes.
  Background: CHEK2(checkpoint kinase 2), also called CHK2 or RAD53, is a cell cycle checkpoint regulator and putative tumor suppressor which contains a forkhead-associated protein interaction domain essential for activation in response to DNA damage and is rapidly phosphorylated in response to replication blocks and DNA damage. CHEK2 is mapped on 22q12.1. CHEK2 was modified by phosphorylation and activated in response to ionizing radiation, and was also modified in response to hydroxyurea treatment. In response to DNA damage, CHEK2 stabilizes the p53 tumor suppressor protein leading to cell cycle arrest in G1. Oligomerization of CHEK2 increases the efficiency of transautophosphorylation, resulting in the release of active CHEK2 monomers that proceed to enforce checkpoint control in irradiated cells. Following gamma irradiation, CHEK2 phosphorylated PML on ser117, causing dissociation of the 2 proteins.
• Reconstitution and Storage: Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml.. At -20C for one year from date of receipt. After reconstitution, at 4C for one month. It can also be aliquotted and stored frozen at -20C for six months. Avoid repeated freezing and thawing.
• Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human Chk2(133-148aa TDKYRTYSKKHFRIFR), identical to the related rat and mouse sequences.
• Purification: Immunogen affinity purified.
• Predicted Homology Based on Immunogen Sequence: Human; Mouse; Rat
• Concentration: 0.1-0.5ug/ml
• Specificity: No cross reactivity with other proteins.
• Application Info: Western blot: 0.1-0.5 ug/ml: Human, Rat: Mouse
• Reference: 1. Ahn, J.-Y., Li, X., Davis, H. L., Canman, C. E. Phosphorylation of threonine 68 promotes
  oligomerization and autophosphorylation of the Chk2 protein kinase via the forkhead-associated
  domain. J. Biol. Chem. 277: 19389-19395, 2002.
  2. Bartkova, J., Horejsi, Z., Koed, K., Kramer, A., Tort, F., Zieger, K., Guldberg, P., Sehested, M.,
  Nesland, J. M., Lukas, C., Orntoft, T., Lukas, J., Bartek, J. DNA damage response as a candidate
  anti-cancer barrier in early human tumorigenesis. Nature 434: 864-870, 2005.
  3. Bell, D. W., Varley, J. M., Szydlo, T. E., Kang, D. H., Wahrer, D. C. R., Shannon, K. E., Lubratovich,
  M., Verselis, S. J., Isselbacher, K. J., Fraumeni, J. F., Birch, J. M., Li, F. P., Garber, J. E., Haber, D.
  A. Heterozygous germ line hCHK2 mutations in Li-Fraumeni syndrome. Science 286: 2528-2531,
  1999.
• Storage Buffer: Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
• Description: Rabbit IgG polyclonal antibody for Serine/threonine-protein kinase Chk2(CHEK2) detection. Tested with WB in Human;Mouse;Rat.

Target Info

• Gene Symbol: CHEK2
• Gene Full Name: checkpoint kinase 2
• Alias Symbols: bA444G7, CDS 1, CDS1, Checkpoint kinase 2, Checkpoint like protein CHK2, Chek 2, Chek2, Chk 2, CHK2 checkpoint homolog(S. pombe), CHK2 checkpoint homolog, CHK2_HUMAN, HuCds 1, HuCds1, LFS 2, LFS2, PP1425, RAD 53, RAD53, Rad53 homolog, Serine/threonine protein kinase Chk2, Serine/threonine-protein kinase Chk2
• Protein Name: Serine/threonine-protein kinase Chk2
• Uniprot ID: O96017