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- $55: Antibody & Protein in US
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Datasheets/Manuals | Printable datasheet for anti-CHEK2 (OASG01552) antibody |
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Predicted Species Reactivity | Human, Mouse, Rat, Monkey |
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Product Format | Liquid. 1 mg/mL in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
Clonality | Polyclonal |
Isotype | IgG |
Host | Rabbit |
Application | WB, IF |
Reconstitution and Storage | Store at -20C. Avoid repeated freeze/thaw cycles. |
Immunogen | Synthesized peptide derived from human Chk2 around the phosphorylation site of T383. Location: 330-410aa. |
Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
Concentration | 1 mg/ml |
Target Post-Translational Modification | Phospho-Thr383 |
Specificity | Phospho-Chk2 (T383) Polyclonal Antibody detects endogenous levels of Chk2 protein only when phosphorylated at T383. |
Application Info | WB 1:500-1:2000; IF 1:200-1:1000; ELISA 1:20000; |
Gene Symbol | CHEK2 |
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Gene Full Name | checkpoint kinase 2 |
Alias Symbols | CDS1, CHK2, LFS2, RAD53, hCds1, HuCds1, PP1425 |
NCBI Gene Id | 11200; 50883; |
Description of Target | In response to DNA damage and replication blocks, cell cycle progression is halted through the control of critical cell cycle regulators. The protein encoded by this gene is a cell cycle checkpoint regulator and putative tumor suppressor. It contains a forkhead-associated protein interaction domain essential for activation in response to DNA damage and is rapidly phosphorylated in response to replication blocks and DNA damage. When activated, the encoded protein is known to inhibit CDC25C phosphatase, preventing entry into mitosis, and has been shown to stabilize the tumor suppressor protein p53, leading to cell cycle arrest in G1. In addition, this protein interacts with and phosphorylates BRCA1, allowing BRCA1 to restore survival after DNA damage. Mutations in this gene have been linked with Li-Fraumeni syndrome, a highly penetrant familial cancer phenotype usually associated with inherited mutations in TP53. Also, mutations in this gene are thought to confer a predisposition to sarcomas, breast cancer, and brain tumors. This nuclear protein is a member of the CDS1 subfamily of serine/threonine protein kinases. Several transcript variants encoding different isoforms have been found for this gene. |
Uniprot ID | O96017, Q9Z265 |
Molecular Weight | 60 kDa |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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What is the species homology for "CHEK2 Antibody (Phospho-Thr383) (OASG01552)"?
The tested species reactivity for this item is "". This antibody is predicted to have homology to "Human, Mouse, Rat, Monkey".
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How long will it take to receive "CHEK2 Antibody (Phospho-Thr383) (OASG01552)"?
This item is available "Domestic: within 2-3 weeks delivery | International: 2-3 weeks".
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What buffer format is "CHEK2 Antibody (Phospho-Thr383) (OASG01552)" provided in?
This item is provided in "Liquid. 1 mg/mL in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "CHEK2 Antibody (Phospho-Thr383) (OASG01552)"?
This target may also be called "CDS1, CHK2, LFS2, RAD53, hCds1, HuCds1, PP1425" in publications.
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What is the shipping cost for "CHEK2 Antibody (Phospho-Thr383) (OASG01552)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
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What is the guarantee for "CHEK2 Antibody (Phospho-Thr383) (OASG01552)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
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Can I get bulk pricing for "CHEK2 Antibody (Phospho-Thr383) (OASG01552)"?
You can get bulk pricing for this item by going here.
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What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "60 kDa".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "CHEK2 Antibody (Phospho-Thr383) (OASG01552)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
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What are positive controls for "CHEK2"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What are negative controls for "CHEK2"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What other proteins interact with "CHEK2"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
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What biological processes are associated with "CHEK2"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
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What cellular components are associated with "CHEK2"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
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What protein functions are associated with "CHEK2"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.