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CHD7 Antibody (OAEB00680)

100 ug
$349.00
In Stock
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Predicted Species Reactivity:
Cow, Guinea Pig, Horse, Human, Mouse, Pig, Rabbit, Rat
Product Format:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Clonality:
Polyclonal
Host:
Goat
Application:
ELISA
Reconstitution and Storage:
Aliquot and store at -20C. Minimize freezing and thawing.
Gene Symbol:
CHD7
NCBI Gene Id:
55636
Protein Name:
Chromodomain-helicase-DNA-binding protein 7
Swissprot Id:
Q9P2D1
Protein Accession #:
NP_060250.2
Alias Symbols:
chromodomain helicase DNA binding protein 7, FLJ20357, FLJ20361, IS3, KIAA1416, Scoliosis, idiopathic 3
Replacement Item:
This antibody may replace item sc-390742 from Santa Cruz Biotechnology.
Tissue Tool:
Find tissues and cell lines supported by DNA array analysis to express CHD7.
RNA Seq:
Find tissues and cell lines supported by RNA-seq analysis to express CHD7.
Predicted Homology Based on Immunogen Sequence:
Cow: 92%; Guinea Pig: 100%; Horse: 92%; Human: 100%; Mouse: 92%; Pig: 92%; Rabbit: 100%; Rat: 92%
Complete computational species homology data:
CHD7 Antibody (OAEB00680)
Peptide Sequence:
C-KDEIDEFANSPSE
Datasheets/Manuals:
Printable datasheet for anti-CHD7 antibody - OAEB00680
Purity:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Application Info:
Peptide ELISA: antibody detection limit dilution 1:64000.
Western Blot: Preliminary experiments gave bands at approx 250kDa and 48kDa in Rat Spinal Cord lysates after 1ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 336kDa according to Human NP_060250.2 and 175kDa according to predicted Rat NP_001101376.1. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands).
Target Reference:
* Gao X, Gordon D, Zhang D, Browne R, Helms C, Gillum J, Weber S, Devroy S, Swaney S, Dobbs M, Morcuende J, Sheffield V, Lovett M, Bowcock A, Herring J, Wise C.; CHD7 gene polymorphisms are associated with susceptibility to idiopathic scoliosis.; Am J Hum Genet. 2007 May;80(5):957-65. Epub 2007 Mar 12.; Pubmed 17436250

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