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Catalog No: OKEI00162
Size:96 Wells
Price: $625.00
SKU
OKEI00162
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHuman
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology CHD5 ELISA Kit (Human) (OKEI00162) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CHD5 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CHD5 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of a luminol substrate which is catalyzed by the HRP to produce light emission. The light emission is read by a luminometer (or photo-multiplier equipped instrument) and the intensity of the emitted light is proportional to the amount of sample CHD5
ELISA Kit Range0.019 - 1 ng/mL
ELISA Kit ReproducibilityIntra-assay reproducibility was evaluated using 20 replicates of 3 samples representing low, middle and high level CHD5. Inter-assay reproducibility was evaluated with 3 samples representing low, middle and high level CHD5 using 8 replicates on each of 3 plates.
Intra-Assay CV <10%
Inter-Assay CV <12%
ELISA Kit Component
ComponentAmount
CHD5 Microplate96 Wells (12 x 8 Well strips)
CHD5 Lyophilized Standard2
100X CHD5 HRP-Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 10 mL
Conjugate Diluent1 x 10 mL
30X Wash Buffer1 x 30 mL
100X Luminol Substrate1 x 10 mL
Substrate Diluent1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Biological Fluids
Sensitivity11.25 pg/mL
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolCHD5
Alias SymbolsCHD-5
NCBI Gene Id26038
Protein NameChromodomain-helicase-DNA-binding protein 5
Description of TargetChromatin-remodeling protein that binds DNA through histones and regulates gene transcription. May specifically recognize and bind trimethylated 'Lys-27' (H3K27me3) and non-methylated 'Lys-4' of histone H3. Plays a role in the development of the nervous system by activating the expression of genes promoting neuron terminal differentiation. In parallel, it may also positively regulate the trimethylation of histone H3 at 'Lys-27' thereby specifically repressing genes that promote the differentiation into non-neuronal cell lineages. Tumor suppressor, it regulates the expression of genes involved in cell proliferation and differentiation. Downstream activated genes may include CDKN2A that positively regulates the p53/TP53 pathway, which in turn, prevents cell proliferation. In spermatogenesis, it probably regulates histone hyperacetylation and the replacement of histones by transition proteins in chromatin, a crucial step in the condensation of spermatid chromatin and the production of functional spermatozoa.
Uniprot IDQ8TDI0
Protein Accession #NP_056372
Nucleotide Accession #NM_015557
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