CHD3 ELISA Kit (Human) (OKEI00161)

Catalog No: OKEI00161

Size:96 Wells

Human CHD3 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Human
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva Systems Biology CHD3 ELISA Kit (Human) (OKEI00161) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CHD3 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CHD3 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of a luminol substrate which is catalyzed by the HRP to produce light emission. The light emission is read by a luminometer (or photo-multiplier equipped instrument) and the intensity of the emitted light is proportional to the amount of sample CHD3
• ELISA Kit Range: 0.313 - 20 ng/mL
• ELISA Kit Reproducibility: Intra-assay reproducibility was evaluated using 20 replicates of 3 samples representing low, middle and high level CHD3. Inter-assay reproducibility was evaluated with 3 samples representing low, middle and high level CHD3 using 8 replicates on each of 3 plates.
  Intra-Assay CV <10%
  Inter-Assay CV <12%
• ELISA Kit Component:
  • CHD3 Microplate: 96 Wells (12 x 8 Well strips)
  • CHD3 Lyophilized Standard: 2
  • 100X CHD3 HRP-Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 10 mL
  • Conjugate Diluent: 1 x 10 mL
  • 30X Wash Buffer: 1 x 30 mL
  • 100X Luminol Substrate: 1 x 10 mL
  • Substrate Diluent: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Biological Fluids
• Sensitivity: 0.188 ng/mL
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: CHD3
• Alias Symbols: ZFH, Mi-2a, SNIBCPS, Mi2-ALPHA
• NCBI Gene Id: 1107
• Protein Name: Chromodomain-helicase-DNA-binding protein 3
• Description of Target: Component of the histone deacetylase NuRD complex which participates in the remodeling of chromatin by deacetylating histones. Required for anchoring centrosomal pericentrin in both interphase and mitosis, for spindle organization and centrosome integrity.
• Uniprot ID: Q12873
• Protein Accession #: NP_001005271
• Nucleotide Accession #: NM_001005271