Catalog No: OCOA09110
Size:20UG
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| Datasheets/Manuals | Printable datasheet for CHAT Protein Lysate (OCOA09110) |
|---|
| Predicted Species Reactivity | Homo sapiens |
|---|---|
| Product Format | Lyophilized |
| Host | HEK293T |
| Additional Information | Components: 1 vial of 20 ug lyophilized gene specific transient over-expression cell lysate |
| :: | Preparation: HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins. |
| Reconstitution and Storage | The lysate can be shipped at ambient temperature. Upon receiving, store the sample at -20C. Lysate samples are stable for 12 months from date of receipt when stored at -20C. Avoid repeated freeze-thaw cycles after reconstitution. Lysate samples can be reconstituted with SDS Sample Buffer. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes |
| Tag | C-MYC/DDK |
| Description | CHAT HEK293T cell transient overexpression lysate (as WB positive control) |
| Gene Symbol | CHAT |
|---|---|
| Gene Full Name | choline O-acetyltransferase |
| Alias Symbols | acetyl CoA:choline O-acetyltransferase;CHOACTASE;choline acetylase;choline O-acetyltransferase;CMS1A;CMS1A2;CMS6. |
| NCBI Gene Id | 1103 |
| Protein Name | A2BDF5, NM_020549, NP_065574, P28329 |
| Description of Target | Choline O-acetyltransferase, choline O-acetyltransferase isoform 2 [Homo sapiens], Homo sapiens choline O-acetyltransferase (CHAT), transcript variant M, mRNA |
| Uniprot ID | https://www.uniprot.org/uniprot/?query=A2BDF5+OR+P28329 |
| Protein Accession # | https://www.ncbi.nlm.nih.gov/protein/NP_065574 |
| Nucleotide Accession # | https://www.ncbi.nlm.nih.gov/nuccore/NM_020549 |
| Molecular Weight | 82.4 kDa |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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