Chapsyn-110 Antibody : FITC (OASE00173-FITC)

Catalog No: OASE00173-FITC

Size:100ug

Product Info

• Predicted Species Reactivity: Human, Mouse, Rat
• Product Format: Liquid. Purified antibody supplied in 1x PBS buffer.
• Clonality: Monoclonal
• Clone: S18-30
• Isotype: IgG1
• Host: Mouse
• Conjugation: FITC: Fluorescein Isothiocyanate
• Application: WB, IHC, IP
• Additional Information: Background Info: Detects ~110kDa. No cross-reactivity against other MAGUK family members, PSD95, SAP97, SAP102, expressed in transfected cells.
  Scientific Background: Chapsyn-110, is a part of the membrane-associated putative guanylate kinase (MAGUK) family. It binds directly to the NMDA receptor, and Shaker K+ channel subunits, and is 70-80% identical to PSD-95/SAP90 and SAP97 (1). It associated tightly with the postsynaptic density I brain, and mediates the clustering of both NMDA recpetors and K+ channels in heterologous cells. The encoded protein forms a heterodimer with PSD-95 that may interact at postsynaptic sites to form a multimeric scaffold for the clustering of receptors, ion channels, and associated signaling proteins (1, 2).
  Certificate of Analysis: 1 ug/mL was sufficient for detection of Chapsyn -110 in 10ug of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
• Reconstitution and Storage: All conjugated antibodies should be stored in light-protected vials or covered with a light protecting material (i.e. aluminum foil). Conjugated antibodies are stable for at least 12 months at 4C. If longer storage is desired (24 months), conjugates may be diluted with up to 50% glycerol and stored at -20C to -80C. Freezing and thawing conjugated antibodies will compromise enzyme activity as well as antibody binding.
• Immunogen: Fusion protein amino acids 1-852 of rat Chapsyn-110; accession number Q63622
• Purification: Protein G Purified
• Concentration: 1 mg/ml
• Dilution: 1-10ug/mL (WB), 0.1-1.0ug/mL (Perox) (IHC/ICC), 1.0-10ug/mL (IF)
• Storage Buffer: PBS pH7.4, 50% glycerol
• Reference: 1. Kim E., et al. (1996) Neuron. 17:103-113.
  2. Godreau D., Neyroud N., Vranckx R. and Hatem S.
  (2004) Med Sci (Paris). 20(1): 84-88.

Target Info

• Gene Symbol: Dlg2
• Gene Full Name: discs, large homolog 2 (Drosophila)
• Alias Symbols: Dlgh2
• NCBI Gene Id: 64053
• Uniprot ID: Q63622
• Protein Accession #: NP_071618.1