CHAF1B ELISA Kit (Mouse) (OKCD09198)

Catalog No: OKCD09198

Size:96WELLS

CHAF1B ELISA Kit (Mouse) (OKCD09198) Standard Curve

Product Info

• Predicted Species Reactivity: Mouse|Mus musculus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Chromatin Assembly Factor 1, Subunit B (CHAF1B). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Chromatin Assembly Factor 1, Subunit B (CHAF1B). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Chromatin Assembly Factor 1, Subunit B (CHAF1B), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Chromatin Assembly Factor 1, Subunit B (CHAF1B) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Chromatin Assembly Factor 1, Subunit B (CHAF1B) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Chromatin Assembly Factor 1, Subunit B (CHAF1B) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • CHAF1B Microplate: 96 Wells (12 x 8 Well strips)
  • CHAF1B Lyophilized Standard: 2
  • 100X CHAF1B HRP-Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 2 mL
  • Stop Solution: 1 x 20 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Tissue homogenates, cell lysates and other biological fluids.
• Sensitivity: < 0.052ng/mL

Target Info

• Gene Symbol: Chaf1b
• Gene Full Name: chromatin assembly factor 1, subunit B (p60)
• Alias Symbols: 2600017H24Rik;C76145;CAF;CAF1;CAF-1 subunit B;CAF1A;CAF1P;CAF1P60;CAF-I;CAF-I 60 kDa subunit;CAF-I p60;CAF-Ip60;chromatin assembly factor 1 subunit B;chromatin assembly factor I p60 subunit;MPHO;MPHOSPH7.
• NCBI Gene Id: 110749
• Protein Name: Chromatin assembly factor 1 subunit B
• Description of Target: Complex that is thought to mediate chromatin assembly in DNA replication and DNA repair. Assembles histone octamers onto replicating DNA in vitro. CAF-1 performs the first step of the nucleosome assembly process, bringing newly synthesized histones H3 and H4 to replicating DNA; histones H2A/H2B can bind to this chromatin precursor subsequent to DNA replication to complete the histone octamer (By similarity).
• Uniprot ID: Q9D0N7
• Protein Accession #: NP_082359.1
• Nucleotide Accession #: NM_028083.4