CHAF1B Antibody - middle region (OASG00939)

Catalog No: OASG00939

Size:100 uL

Product Info

• Predicted Species Reactivity: Human, Mouse, Rat
• Product Format: Liquid. PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
• Clonality: Polyclonal
• Isotype: IgG
• Host: Rabbit
• Application: WB, IHC-P, IF, ELISA
• Additional Information: Cellular Location: Nucleus, Cytoplasm, DNA Replication Foci, Cytoplasmic in M Phase
• Reconstitution and Storage: Store at -20°C. Avoid repeated freeze/thaw cycles.
• Immunogen: Synthesized peptide derived from the Internal region of human CAF-1 p60. Location: 40-120aa.
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using a epitope-specific immunogen.
• Concentration: 1 mg/ml
• Target Post-Translational Modification: Differentially phosphorylated during cell cycle. During mitosis the p60 subunit of inactive CAF-1 is hyperphosphorylated and displaced into the cytosol. Progressivly dephosphorylated from G1 to S and G2 phase. Phosphorylated p60 is recruited to chromatin undergoing DNA repair after UV irradiation in G1, S or G2 phases.
• Specificity: CAF-1 p60 Polyclonal Antibody detects endogenous levels of CAF-1 p60 protein.
• Application Info: WB 1:500~2000
  IHC 1:100~300
  IF 1:200~1000
  ELISA 1:20000

Target Info

• Gene Symbol: CHAF1B
• Gene Full Name: chromatin assembly factor 1, subunit B (p60)
• Alias Symbols: CAF1, MPP7, CAF-1, CAF1A, CAF1P60, CAF-IP60, MPHOSPH7
• NCBI Gene Id: 8208; 110749;
• Description of Target: Complex that is thought to mediate chromatin assembly in DNA replication and DNA repair. Assembles histone octamers onto replicating DNA in vitro. CAF-1 performs the first step of the nucleosome assembly process, bringing newly synthesized histones H3 and H4 to replicating DNA; histones H2A/H2B can bind to this chromatin precursor subsequent to DNA replication to complete the histone octamer.
• Uniprot ID: Q13112, Q9D0N7