CHAD ELISA Kit (Bovine) (OKEH08109)

Catalog No: OKEH08109

Size:96T

Product Info

• Predicted Species Reactivity: Bos taurus, Bovine
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Principle: Aviva Systems Biology CHAD ELISA Kit (Bovine) (OKEH08109) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CHAD has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CHAD is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample CHAD captured in well.
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 113% (n = 20)
• ELISA Kit Reproducibility: Mean Intra-assay CV%: <=2.7% (n = 20)
  Mean Inter-assay CV%: <=7.4% (n = 20)
• ELISA Kit Component:
  • CHAD Microplate: 96 Wells (12 x 8 Well strips)
  • CHAD Lyophilized Standard: 2
  • 100X Biotinylated CHAD Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Additional Information: Function: Promotes attachment of chondrocytes, fibroblasts, and osteoblasts. This binding is mediated (at least for chondrocytes and fibroblasts) by the integrin alpha(2)beta(1). May play an important role in the regulation of chondrocyte growth and proliferation.
  Subcellular Location: Secreted Extracellular space Extracellular matrix
  Unigene: Bt.91648
  String: 9913.ENSBTAP00000028386
  KEGG: bta:281069
  Pfam: PF00560
• Reconstitution and Storage: Store as indicated in product manual.
• Specificity: Natural and recombinant Bovine Chondroadherin
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: CHAD
• Gene Full Name: chondroadherin
• Alias Symbols: 38 kDa bone protein;cartilage leucine-rich protein;chondroadherin.
• NCBI Gene Id: 281069
• Protein Name: Q27972
• Description of Target: Chondroadherin
• Uniprot ID: https://www.uniprot.org/uniprot/Q27972
• Protein Accession #: NP_776444.1
• Nucleotide Accession #: NM_174019.2