cGKI Antibody - C-terminal region (OAAB17360)

Catalog No: OAAB17360

Size:400ul

The anti-cGKI beta Pab (Cat. # OAAB17360) is used in Western blot to detect cGKI beta in 293 cell lysate (Lane 1) and mouse small intestine tissue lysate (Lane 2).

PRKG1 is supported by BioGPS gene expression data to be expressed in HEK293

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

Product Info

• Tested Species Reactivity: Human, Mouse
• Predicted Species Reactivity: Bovine
• Product Format: Liquid PBS with 0.09% (W/V) sodium azide.
• Clonality: Polyclonal
• Isotype: Ig
• Host: Rabbit
• Application: WB, IHC-P, ELISA
  Cellular Location: Cytoplasm. Note=Colocalized with TRPC7 in the plasma membrane.
  Tissue Location: Primarily expressed in lung and placenta.
• Reconstitution and Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: This cGKI (cGKI beta) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 629-660 amino acids from the C-terminal region of human cGKI beta.
• Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.
• Concentration: 0.4 mg/mL
• Sample Type Confirmation:

  PRKG1 is supported by BioGPS gene expression data to be expressed in HEK293

• Application Info: WB: 1:1000
  IHC-P: 1:100
• Protein Range: 629-660

Target Info

• Gene Symbol: PRKG1
• Alias Symbols: PKG, cGK, AAT8, PKG1, cGK1, cGKI, cGK 1, PRKG1B, PRKGR1B, cGKI-BETA, cGKI-alpha
• NCBI Gene Id: 5592
• Protein Name: cGMP-dependent protein kinase 1
• Description of Target: Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains.
• Uniprot ID: Q13976
• Protein Accession #: NP_001091982.1
• Molecular Weight: 76 kDa