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Catalog No: OKCD05587
Size:96WELLS
Price: $850.00
SKU
OKCD05587
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CGB3 MORE Chemi-Luminescent ELISA Kit (Human) (OKCD05587)

Datasheets/ManualsPrintable datasheet for CGB3 MORE Chemi-Luminescent ELISA Kit (Human) (OKCD05587)
Product Info
Predicted Species ReactivityHomo sapiens|Human
ApplicationChemiluminescent immunoassay
ELISA Kit Detection MethodChemiluminescent
ELISA Kit Duration2h, 40min
ELISA Kit PrincipleThe microplate provided in this kit has been pre-coated with an antibody specific to Chorionic Gonadotropin (CG). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Chorionic Gonadotropin (CG). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Chorionic Gonadotropin (CG) level in the sample or standard.
ELISA Kit Range13.7-10,000pg/mL
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Chorionic Gonadotropin (CG) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Chorionic Gonadotropin (CG) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
CGB3 MORE Microplate96 Wells (12 x 8 Well strips)
CGB3 MORE Lyophilized Standard2
100X CGB3 MORE HRP-Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
100X Luminol Substrate1 x 9 mL
Substrate Diluent1 x 6 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma, urine, cell culture supernates and other biological fluids
Sensitivity< 5.1pg/mL
Gene SymbolCGB3|CGB5|CGB8
Gene Full Namechorionic gonadotropin subunit beta 3|chorionic gonadotropin subunit beta 5|chorionic gonadotropin subunit beta 8
Alias SymbolsCGB;CGB5;CGB7;CGB8;CG-beta;Choriogonadotropin subunit beta;choriogonadotropin subunit beta 3;chorionic gonadotrophin chain beta;chorionic gonadotropin beta 3 subunit;chorionic gonadotropin beta 5 subunit;chorionic gonadotropin beta 8 subunit;chorionic gonadotropin beta chain;chorionic gonadotropin beta subunit 3;chorionic gonadotropin beta subunit 5;chorionic gonadotropin beta subunit 8;chorionic gonadotropin chain beta;chorionic gonadotropin, beta polypeptide;chorionic gonadotropin, beta polypeptide 5;chorionic gonadotropin, beta polypeptide 8;HCG;hCGB;LHB;luteinizing hormone beta subunit.
NCBI Gene Id1082|93659|94115
Protein NameChoriogonadotropin subunit beta 3
Description of TargetBeta subunit of the human chorionic gonadotropin (hCG). hCG is a complex glycoprotein composed of two glycosylated subunits alpha and beta which are non-covalently associated. The alpha subunit is identical to those in the pituitary gonadotropin hormones (LH, FSH and TSH). The beta subunits are distinct in each of the hormones and confer receptor and biological specificity. Has an essential role in pregnancy and maternal adaptation. Stimulates the ovaries to synthesize the steroids that are essential for the maintenance of pregnancy.
Uniprot IDP0DN86
Protein Accession #NP_000728.1
Nucleotide Accession #NM_000737.3
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