Catalog No: OKEH00451
Size:96 Wells
Price: $525.00
SKU
OKEH00451
Availability: Domestic: within 2-3 weeks delivery | International: 2-3 weeks
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CGB ELISA Kit (Human) : 96 Wells (OKEH00451)

Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHuman
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology CGB ELISA Kit (Human) (OKEH00451) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Human CGB has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. After washing,a biotinylated detector antibody specific for Human CGB is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Human CGB captured in well.
ELISA Kit Range0.78 - 50ng/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 96%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 3.9% (n = 20)
Mean Inter-assay CV%: < 7.3% (n = 20)
ELISA Kit Component
ComponentAmount
Anti-CGB Microplate96 Wells (12 x 8 Well strips)
CGB Lyophilized Standard2
Sample Diluent1 x 20 mL
100X Biotinylated CGB1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Sensitivity0.35 ng/mL
SpecificityNatural and recombinant Human Choriogonadotropin subunit beta
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolCGB
Gene Full Namechorionic gonadotropin beta subunit 3
Alias SymbolsCGB, LHB, CGB5, CGB7, CGB8, hCGB
NCBI Gene Id1082
Protein Namechorionic gonadotropin, beta polypeptide 7
Description of TargetStimulates the ovaries to synthesize the steroids that are essential for the maintenance of pregnancy.
Swissprot IdP0DN86
Protein Accession #NP_000728
Nucleotide Accession #NM_000737
Protein Size (# AA)165
Molecular Weight17 kDa
Tissue ToolFind tissues and cell lines supported by DNA array analysis to express CGB.
RNA SeqFind tissues and cell lines supported by RNA-seq analysis to express CGB.
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