Catalog No: OKEH03760
Size:96T
Price: $375.00
SKU
OKEH03760
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityBos taurus, Bovine
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology CGA ELISA Kit (Bovine) (OKEH03760) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CGA has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CGA is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample CGA captured in well.
ELISA Kit Range0.78-50mIU/mL
ELISA Kit Component
ComponentAmount
Anti-CGA Microplate96 Wells (12 x 8 Well strips)
CGA Lyophilized Standard2
Sample Diluent1 x 20 mL
100X Biotinylated CGA Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Sensitivity0.22mIU/mL
SpecificityNatural and recombinant Bovine Glycoprotein hormones alpha chain
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolCGA
Gene Full Nameglycoprotein hormones, alpha polypeptide
Alias Symbolsanterior pituitary glycoprotein hormones common subunit alpha;chorionic gonadotropin, alpha chain;follicle-stimulating hormone alpha chain;follitropin alpha chain;FSH-alpha;glycoprotein hormones alpha chain;LSH-alpha;luteinizing hormone alpha chain;lutropin alpha chain;thyroid-stimulating hormone alpha chain;thyrotropin alpha chain;TSH-alpha.
NCBI Gene Id280749
Protein NameP01217
Description of TargetGlycoprotein hormones alpha chain
Uniprot IDhttps://www.uniprot.org/uniprot/P01217
Protein Accession #NP_776326.1
Nucleotide Accession #NM_173901.3
Protein Size (# AA)120
Molecular Weight14 kDa
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