CFTR Antibody (OAAF00999)

Catalog No: OAAF00999 (Formerly GWB-ASC404)

Size:100 ug

Western blot analysis of extracts from NIH-3T3 cells, using CFTR Antibody. The lane on the right is treated with the synthesized peptide.

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue, using CFTR Antibody. The picture on the right is treated with the synthesized peptide.

Product Info

• Predicted Species Reactivity: Human|Mouse|Rat
• Product Format: Liquid
• Clonality: Polyclonal
• Host: Rabbit
• Application: Enzyme-linked immunosorbent assay|Immunofluorescence|Immunohistochemistry|Immunohistochemistry-Paraffin|Western blot
• Reconstitution and Storage: -20°C
• Immunogen: The antiserum was produced against synthesized peptide derived from human CFTR.
• Purification: The antibody was purified from rabbit antiserum by affinity-chromatography using immunogen.
• Specificity: CFTR antibody detects endogenous levels of total CFTR protein.
• Formulation: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Dilution: The optimal working dilution should be determined by the end user.
• Application Info: WB: 1:500~1:1000; IHC: 1:50~1:100; ELISA: 1:1000
• Reference: Marko Bertog, Am J Physiol Cell Physiol, May 2000; 278: C885 - C894.
  H. Kopelman, Am J Physiol Cell Physiol, Sep 1995; 269: C626 - C631.
  Meshell Johnson, FASEB J, Mar 2006; 20: A746.
  Albert Tousson, Am J Physiol Cell Physiol, Dec 1998; 275: C1555 - C1564

Target Info

• Gene Symbol: CFTR
• Gene Full Name: CF transmembrane conductance regulator
• Alias Symbols: ABC35;ABCC7;ATP-binding cassette sub-family C member 7;cAMP-dependent chloride channel;CF;CFTR/MRP;channel conductance-controlling ATPase;cystic fibrosis transmembrane conductance regulating;cystic fibrosis transmembrane conductance regulator;cystic fibrosis transmembrane conductance regulator (ATP-binding cassette sub-family C, member 7);dJ760C5.1;MRP7;TNR-CFTR.
• NCBI Gene Id: 1080
• Protein Name: Cystic fibrosis transmembrane conductance regulator
• Description of Target: Epithelial ion channel that plays an important role in the regulation of epithelial ion and water transport and fluid homeostasis (PubMed:26823428). Mediates the transport of chloride ions across the cell membrane (PubMed:10792060, PubMed:11524016, PubMed:11707463, PubMed:12519745, PubMed:15010471, PubMed:12588899, PubMed:17036051, PubMed:19398555, PubMed:19621064, PubMed:22178883, PubMed:25330774, PubMed:1712898, PubMed:8910473, PubMed:9804160, PubMed:12529365, PubMed:17182731, PubMed:26846474, PubMed:28087700). Channel activity is coupled to ATP hydrolysis (PubMed:8910473). The ion channel is also permeable to HCO(3-); selectivity depends on the extracellular chloride concentration (PubMed:15010471, PubMed:19019741). Exerts its function also by modulating the activity of other ion channels and transporters (PubMed:12403779, PubMed:22178883, PubMed:22121115, PubMed:27941075). Plays an important role in airway fluid homeostasis (PubMed:16645176, PubMed:19621064, PubMed:26823428). Contributes to the regulation of the pH and the ion content of the airway surface fluid layer and thereby plays an important role in defense against pathogens (PubMed:14668433, PubMed:16645176, PubMed:26823428). Modulates the activity of the epithelial sodium channel (ENaC) complex, in part by regulating the cell surface expression of the ENaC complex (PubMed:17434346, PubMed:27941075, PubMed:17182731). Inhibits the activity of the ENaC channel containing subunits SCNN1A, SCNN1B and SCNN1G (PubMed:17182731). Inhibits the activity of the ENaC channel containing subunits SCNN1D, SCNN1B and SCNN1G, but not of the ENaC channel containing subunits SCNN1A, SCNN1B and SCNN1G (PubMed:27941075). May regulate bicarbonate secretion and salvage in epithelial cells by regulating the transporter SLC4A7 (PubMed:12403779). Can inhibit the chloride channel activity of ANO1 (PubMed:22178883). Plays a role in the chloride and bicarbonate homeostasis during sperm epididymal maturation and capacitation (PubMed:19923167, PubMed:27714810).
• Uniprot ID: P13569
• Protein Accession #: NP_000483.3
• Molecular Weight: 168 kDa