CFSE (OOTA00658)

Catalog No: OOTA00658

Size:500 ug

Human peripheral blood mononuclear cells were labeled with CFSE (OOTA00658) and left unstimulated (left panel) or stimulated for 4 days with Anti-Human CD3 and Anti-Human CD28 (right panel). Plots show intensity of CFSE label vs. staining with APC Anti-Hu

Product Info

• Product Format: Lyophilized
• Application: FC
• Reconstitution and Storage: -20C protected from light and moisture
• Application Info: CFSE ( 5-(and -6)-Carboxyfluorescein diacetate, succinimidyl ester) has been quality-tested for flow cytometry using stimulated human peripheral blood mononuclear cells. CFSE can be used to label cells at concentrations ranging from 0.5-20 uM, depending on the cell type and assay. It is recommended that the investigator determine the optimal concentration of CFSE for the assay of interest. Over-labeling of cells with high concentrations of CFSE can obstruct normal cell functions and interfere with compensation in multicolor experiments.
• Reference:

  Lyons AB, Parish CR. 1994. J Immunol Methods. 171(1):131-137.

  Lyons AB. 2000. J Immunol Methods. 243(1-2):147-154.

  Miller MJ, Wei SH, Parker I, Cahalan MD. 2002. Science. 296(5574):1869-1873.

  Parish CR, Glidden MH, Quah BJ, Warren HS. 2009. Curr Protoc Immunol. Chapter 4:Unit4.9.

Target Info

• Alias Symbols: 5-(and -6)-Carboxyfluorescein diacetate succinimidyl ester