Catalog No: OKEH04438
Size:96 Wells
Price: $525.00
SKU
OKEH04438
Availability: Domestic: within 2-3 weeks delivery | International: 2-3 weeks
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHuman
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology CFP ELISA Kit (Human) (OKEH04438) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CFP has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. After washing, a biotinylated detector antibody specific for CFP is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes to yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample CFP captured in the well.
ELISA Kit Range0.156 - 10ng/ml
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 89%
ELISA Kit ReproducibilityMean Intra-assay CV%: <= 3.2% (n = 20)
Mean Inter-assay CV%: <= 6.4% (n = 20)
ELISA Kit Component
ComponentAmount
Anti-CFP Microplate96 Wells (12 x 8 Well Strips)
CFP Lyophilized Standard2 x 2 ng
Sample Diluent1 x 20 mL
CFP Biotinylated Detector Antibody1 x 120 uL
Detector Antibody Diluent1 x 12 mL
HRP Avidin Conjugate1 x 120 uL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Sensitivity0.079 ng/mL
SpecificityNatural and recombinant Human Properdin
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolCFP
Gene Full Namecomplement factor properdin
Alias SymbolsBFD, PFC, PFD, PROPERDIN
NCBI Gene Id5199
Protein NameProperdin
Description of TargetThis gene encodes a plasma glycoprotein that positively regulates the alternative complement pathway of the innate immune system. This protein binds to many microbial surfaces and apoptotic cells and stabilizes the C3- and C5-convertase enzyme complexes in a feedback loop that ultimately leads to formation of the membrane attack complex and lysis of the target cell. Mutations in this gene result in two forms of properdin deficiency, which results in high susceptibility to meningococcal infections. Multiple alternatively spliced variants, encoding the same protein, have been identified.
Swissprot IdP27918
Protein Accession #NP_001138724.1
Nucleotide Accession #NM_001145252.1
Protein Size (# AA)469
Molecular Weight51 KDa
Tissue ToolFind tissues and cell lines supported by DNA array analysis to express CFP.
RNA SeqFind tissues and cell lines supported by RNA-seq analysis to express CFP.
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