Catalog No: OKCD06813
Size:96 Wells
Price: $600.00
SKU
OKCD06813
Availability: Domestic: within 2-3 weeks delivery | International: 2-3 weeks
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHuman
ApplicationELISA
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology CFP ELISA Kit (Human) (OKCD06813) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CFP has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CFP is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample CFP captured in the well.
ELISA Kit Range15.6-1000ng/mL
ELISA Kit ReproducibilityIntra-assay reproducibility was evaluated using 20 replicates of 3 samples representing low, middle and high level CFP. Inter-assay reproducibility was evaluated with 3 samples representing low, middle and high level CFP using 8 replicates on each of 3 plates.
Intra-Assay CV <10%
Inter-Assay CV <12%
ELISA Kit Component
ComponentAmount
CFP Microplate96 Wells (12 x 8 Well strips)
CFP Lyophilized Standard2
100X CFP HRP-Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, plasma and other biological fluids
Sensitivity< 6.5ng/mL
Gene SymbolCFP
Gene Full Namecomplement factor properdin
Alias SymbolsBFD, PFC, PFD, PROPERDIN
NCBI Gene Id5199
Protein NameProperdin
Description of TargetCFP is a plasma glycoprotein that positively regulates the alternative complement pathway of the innate immune system. This protein binds to many microbial surfaces and apoptotic cells and stabilizes the C3- and C5-convertase enzyme complexes in a feedbac
Swissprot IdP27918
Protein Accession #NP_001138724.1
Nucleotide Accession #NM_001145252.1
Tissue ToolFind tissues and cell lines supported by RNA-seq analysis to express CFP.
RNA SeqFind tissues and cell lines supported by DNA array analysis to express CFP.
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