CFP ELISA Kit (Human) (OKBB01156)

Catalog No: OKBB01156

Size:96 wells

CFP ELISA Kit (Human) (OKBB01156) Standard Curve

Product Info

• Predicted Species Reactivity: Human
• Application: ELISA
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva's human Properdin ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Properdin has been precoated onto 96-well plates. Standards (Expression system for standard: NS0, Immunogen sequence: D28-L469) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Properdin is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the human Properdin amount of sample captured in plate.
• ELISA Kit Range: 312 pg/ml - 20,000 pg/ml
• ELISA Kit Reproducibility:

  	Intra-Assay Precision			Inter-Assay Precision
  Sample	1	2	3	1	2	3
  n	16	16	16	24	24	24
  Mean(pg/ml)	616	2125	9062	602	2285	9683
  Standard deviation	28.33	91.37	561.84	30.1	109.68	629.39
  CV(%)	4.6	4.3	6.2	5	4.8	6.5

• ELISA Kit Component:
  • 96-Well Plate Pre-coated with Anti-Human CFP Antibody: 96 Wells (12 x 8 Well Strips)
  • Lyophilized Recombinant Human CFP Standard: 2 x 10 ng
  • 100X Biotinylated Anti-Human CFP Antibody: 1 x 130 uL
  • 100X Avidin-Biotin Peroxidase Complex (ABC): 1 x 130 uL
  • Sample Diluent Buffer: 1 x 30 mL
  • Antibody Diluent Buffer: 1 x 12 mL
  • ABC Diluent Buffer: 1 x 12 mL
  • TMB Color Developing Agent: 1 x 10 mL
  • TMB Stop Solution: 1 x 10 mL
  • 10X Wash Buffer: 1 x 30 mL
• Additional Information:

  To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.

  Lots	Lot1 (pg/ml)	Lot2 (pg/ml)	Lot3 (pg/ml)	Lot4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
  Sample 1	616	576	554	549	573	26.42	4.6%
  Sample 2	2125	2256	2211	2286	2219	60.73	2.7%
  Sample 3	9062	9597	10032	10437	9782	510.91	5.2%

  *number of samples for each test n=16.

  
  

  Standard Number	8	7	6	5	4	3	2	1
  Concentration (pg/ml)	0	312	625	1250	2500	5000	10000	20000
  O.D.	0.051	0.166	0.249	0.38	0.671	1.241	1.992	2.492

• Reconstitution and Storage: Store at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
• Sample Type: cell culture supernates, serum and plasma (heparin, EDTA)
• Sensitivity: <10 pg/ml
• Specificity: Natural and recombinant human Properdin
• Immunogen: Expression system for standard: NS0; Immunogen sequence: D28-L469
• Description: For Quantitative Detection of human Properdin in cell culture supernatants, serum and plasma (heparin, EDTA).

Target Info

• Gene Symbol: CFP
• Gene Full Name: complement factor properdin
• Alias Symbols: Properdin; Complement factor P; CFP; PFC
• NCBI Gene Id: 5199
• Protein Name: Properdin
• Description of Target: Properdin or Factor P is the only known positive regulator of complement activation that stabilizes thealternative pathway convertases. This gene encodes a plasma glycoprotein that positively regulates the alternative complement pathway of the innate immune system. This protein binds to many microbial surfaces and apoptotic cells and stabilizes the C3- and C5-convertase enzyme complexes in a feedback loop that ultimately leads to formation of the membrane attack complex and lysis of the target cell. Mutations in this gene result in two forms of properdin deficiency, which results in high susceptibility to meningococcal infections. Multiple alternatively spliced variants, encoding the same protein, have been identified.
• Uniprot ID: P27918
• Protein Accession #: NP_001138724.1
• Nucleotide Accession #: NM_001145252.1