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CFLAR Antibody - middle region (OAAB05713)

Catalog#: OAAB05713
Domestic: within 1 week delivery International: 1 week
More Information
Predicted Species Reactivity Human
Product Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Clonality Polyclonal
Isotype Rabbit Ig
Host Rabbit
Application WB, IHC, FC
Reconstitution and Storage Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
Replacement Item This antibody may replace item sc-136160, HPA019044
Immunogen This CFLAR antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 145-174 amino acids from the Central region of human CFLAR.
Predicted Homology Based on Immunogen Sequence Human
Concentration Approximately 0.5mg/ml. Actual concentration varies with each lot.
Datasheets/Manuals Printable datasheet for anti-CFLAR antibody - OAAB05713
Sample Type Confirmation

There is BioGPS gene expression data showing that CFLAR is expressed in HepG2

Application Info WB: 1:1000
IHC-P: 1:50~100
FC: 1:10~50
Other Applications Image 1 Data CFLAR Antibody (Center) flow cytometric analysis of k562 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Albrecht, E. A., Sarma, J. V & Ward, P. A. Activation by C5a of endothelial cell caspase 8 and cFLIP. Inflamm. Res. 58, 30-7 (2009). WB, IHC, FC, ELISA, Human 19115040

Toporkiewicz, M. et al. Release of an ~55kDa fragment containing the actin-binding domain of b-spectrin by caspase-8 during FND-induced apoptosis depends on the presence of protein 4.1. Arch. Biochem. Biophys. 535, 205-13 (2013). WB, IHC, FC, ELISA, Human 23578573

Yajima, T. et al. Resistance to cytotoxic chemotherapy-induced apoptosis in side population cells of human oral squamous cell carcinoma cell line Ho-1-N-1. Int. J. Oncol. 35, 273-80 (2009). WB, IHC, FC, ELISA, Human 19578740

Gene Symbol CFLAR
Alias Symbols CASP8 and FADD-like apoptosis regulator subunit p12, CASP8 and FADD-like apoptosis regulator subunit p43, Usurpin, FADD-like antiapoptotic molecule 1, FLAME-1, Inhibitor of FLICE, I-FLICE, Caspase homolog, CASH, MACH-related inducer of toxicity, MRI
NCBI Gene Id 8837
Protein Name CASP8 and FADD-like apoptosis regulator
Swissprot Id O15519
Protein Accession # NP_001120655.1
Molecular Weight 55344 Da
Tissue Tool Find tissues and cell lines supported by DNA array analysis to express CFLAR.
RNA Seq Find tissues and cell lines supported by RNA-seq analysis to express CFLAR.
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