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CFLAR Antibody - middle region (OAAB05713)

400ul
$320.00
In Stock
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Gene Symbol:
CFLAR
NCBI Gene Id:
8837
Protein Name:
CASP8 and FADD-like apoptosis regulator
Swissprot Id:
O15519
Protein Accession #:
NP_001120655.1
Alias Symbols:
CASP8 and FADD-like apoptosis regulator subunit p12, CASP8 and FADD-like apoptosis regulator subunit p43, Usurpin, FADD-like antiapoptotic molecule 1, FLAME-1, Inhibitor of FLICE, I-FLICE, Caspase homolog, CASH, MACH-related inducer of toxicity, MRI
Replacement Item:
This antibody may replace item sc-136160, HPA019044
Molecular Weight:
55344 Da
Host:
Rabbit
Clonality:
Polyclonal
Application:
WB, IHC, FC, ELISA
Tissue Tool:
Find tissues and cell lines supported by DNA array analysis to express CFLAR.
RNA Seq:
Find tissues and cell lines supported by RNA-seq analysis to express CFLAR.
Immunogen:
This CFLAR antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 152~182 amino acids from the Center region of human CFLAR.
Predicted Homology Based on Immunogen Sequence:
Human
Product Format:
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Reconstitution and Storage:
Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles.
Concentration:
Approximately 0.5mg/ml. Actual concentration varies with each lot.
Datasheets/Manuals:
Printable datasheet for anti-CFLAR antibody - OAAB05713
Sample Type Confirmation:

There is BioGPS gene expression data showing that CFLAR is expressed in HepG2

Isotype:
Rabbit Ig
Application Info:
The suggested dilution is:
ELISA ~~ 1:1,000
Western blotting~~ 1:50~100
Immunohistochemistry ~~ 1:50~100
Flow cytometric~~1:10~50
Publications:

Yajima, T. et al. Resistance to cytotoxic chemotherapy-induced apoptosis in side population cells of human oral squamous cell carcinoma cell line Ho-1-N-1. Int. J. Oncol. 35, 273-80 (2009). WB, IHC, FC, ELISA, Human 19578740

Albrecht, E. A., Sarma, J. V & Ward, P. A. Activation by C5a of endothelial cell caspase 8 and cFLIP. Inflamm. Res. 58, 30-7 (2009). WB, IHC, FC, ELISA, Human 19115040

Toporkiewicz, M. et al. Release of an ~55kDa fragment containing the actin-binding domain of β-spectrin by caspase-8 during FND-induced apoptosis depends on the presence of protein 4.1. Arch. Biochem. Biophys. 535, 205-13 (2013). WB, IHC, FC, ELISA, Human 23578573

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