CFL1 ELISA Kit (Human) (OKCD07490)

Catalog No: OKCD07490

Size:96WELLS

CFL1 ELISA Kit (Human) (OKCD07490) Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cofilin 1 (CFL1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cofilin 1 (CFL1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cofilin 1 (CFL1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Cofilin 1 (CFL1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Cofilin 1 (CFL1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Cofilin 1 (CFL1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • CFL1 Microplate: 96 Wells (12 x 8 Well strips)
  • CFL1 Lyophilized Standard: 2
  • 100X CFL1 HRP-Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 2 mL
  • Stop Solution: 1 x 20 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Tissue homogenates, cell lysates and other biological fluids
• Sensitivity: < 0.060ng/mL

Target Info

• Gene Symbol: CFL1
• Gene Full Name: cofilin 1
• Alias Symbols: 18 kDa phosphoprotein;CFL;cofilin;cofilin 1 (non-muscle);Cofilin, non-muscle isoform;cofilin-1;epididymis secretory protein Li 15;HEL-S-15;p18.
• NCBI Gene Id: 1072
• Protein Name: Cofilin-1
• Description of Target: Binds to F-actin and exhibits pH-sensitive F-actin depolymerizing activity. Regulates actin cytoskeleton dynamics. Important for normal progress through mitosis and normal cytokinesis. Plays a role in the regulation of cell morphology and cytoskeletal organization. Required for the up-regulation of atypical chemokine receptor ACKR2 from endosomal compartment to cell membrane, increasing its efficiency in chemokine uptake and degradation (PubMed:11812157, PubMed:15580268, PubMed:21834987, PubMed:23633677). Required for neural tube morphogenesis and neural crest cell migration (By similarity).
• Uniprot ID: P23528
• Protein Accession #: NP_005498.1
• Nucleotide Accession #: NM_005507.2