CFL1 ELISA Kit (Human) : 96 Wells (OKEH01091)

Catalog No: OKEH01091

Size:96 Wells

Human CFL1 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Human
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva Systems Biology CFL1 ELISA Kit (Human) (OKEH01091) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CFL1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CFL1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample CFL1 captured in well.
• ELISA Kit Range: 0.156 - 10ng/mL
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 109%
• ELISA Kit Reproducibility: Mean Intra-assay CV%: < 6.5% (n = 20)
  Mean Inter-assay CV%: < 10.1% (n = 20)
• ELISA Kit Component:
  • CFL1 Microplate: 96 Wells (12 x 8 Well strips)
  • CFL1 Lyophilized Standard: 2
  • 100X Biotinylated CFL1 Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
• Sensitivity: 0.078 ng/mL
• Specificity: Natural and recombinant Human Cofilin-1
• Publications:

  Tandem Mass Tag-Based Serum Proteome Profiling for Biomarker Discovery in Young Duchenne Muscular Dystrophy Boys. ACS Omega. 5, 26504-26517 (2020). 33110978

• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: CFL1
• Gene Full Name: cofilin 1
• Alias Symbols: CFL, cofilin, HEL-S-15
• NCBI Gene Id: 1072
• Protein Name: Cofilin-1
• Description of Target: The protein encoded by this gene can polymerize and depolymerize F-actin and G-actin in a pH-dependent manner. Increased phosphorylation of this protein by LIM kinase aids in Rho-induced reorganization of the actin cytoskeleton. Cofilin is a widely distributed intracellular actin-modulating protein that binds and depolymerizes filamentous F-actin and inhibits the polymerization of monomeric G-actin in a pH-dependent manner. It is involved in the translocation of actin-cofilin complex from cytoplasm to nucleus.
• Uniprot ID: P23528
• Protein Accession #: NP_005498.1
• Nucleotide Accession #: NM_005507.2
• Protein Size (# AA): 166
• Molecular Weight: 19 kDa