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Catalog No: OKEH01091
Price: $600.00
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHomo sapiens, Human
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology CFL1 ELISA Kit (Human) (OKEH01091) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CFL1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CFL1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample CFL1 captured in well.
ELISA Kit Range0.156-10ng/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 109%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 6.5% (n = 20)
Mean Inter-assay CV%: < 10.1% (n = 20)
ELISA Kit Component
CFL1 Microplate96 Wells (12 x 8 Well strips)
CFL1 Lyophilized Standard2
100X Biotinylated CFL1 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
Sensitivity0.078 ng/mL
SpecificityNatural and recombinant Human Cofilin-1

Tandem Mass Tag-Based Serum Proteome Profiling for Biomarker Discovery in Young Duchenne Muscular Dystrophy Boys. ACS Omega. 5, 26504-26517 (2020). 33110978

Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolCFL1
Gene Full Namecofilin 1
Alias Symbols18 kDa phosphoprotein;CFL;cofilin;cofilin 1 (non-muscle);Cofilin, non-muscle isoform;cofilin-1;epididymis secretory protein Li 15;HEL-S-15;p18.
NCBI Gene Id1072
Protein NameP23528
Description of TargetCofilin-1
Uniprot ID
Protein Accession #NP_005498.1
Nucleotide Accession #NM_005507.2
Protein Size (# AA)166
Molecular Weight19 kDa
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