Catalog No: OKCD07862
Size:96WELLS
Price: $750.00
SKU
OKCD07862
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Datasheets/ManualsPrintable datasheet for CFI ELISA Kit (Human) (OKCD07862)
Product Info
Predicted Species ReactivityHomo sapiens|Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Complement Factor I (CFI). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Complement Factor I (CFI). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Complement Factor I (CFI), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Complement Factor I (CFI) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range3.12-200ng/mL
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Complement Factor I (CFI) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Complement Factor I (CFI) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
CFI Microplate96 Wells (12 x 8 Well strips)
CFI Lyophilized Standard2
100X CFI HRP-Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 2 mL
Stop Solution1 x 20 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma and other biological fluids
Sensitivity< 0.072ng/mL
Gene SymbolCFI
Gene Full Namecomplement factor I
Alias SymbolsAHUS3;ARMD13;C3B/C4B inactivator;C3b-INA;C3BINA;C3b-inactivator;complement component I;complement control protein factor I;complement factor I;complement factor I heavy chain;FI;IF;KAF;Konglutinogen-activating factor;light chain of factor I.
NCBI Gene Id3426
Protein NameComplement factor I
Description of TargetResponsible for cleaving the alpha-chains of C4b and C3b in the presence of the cofactors C4-binding protein and factor H respectively.
Uniprot IDP05156
Protein Accession #NP_000195.2
Nucleotide Accession #NM_000204.4
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