CFHR5 ELISA Kit (Human) (OKCD02042)

Catalog No: OKCD02042 (Formerly GWB-KBBIK2)

Size:96WELLS

Human CFHR5 ELISA Kit Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2 Dilution	1:4 Dilution	1:8 Dilution	1:16 Dilution
  Serum (n=5)	78-104%	79-97%	78-93%	78-103%
  EDTA Plasma (n=5)	83-101%	81-102%	98-105%	89-96%
  Heparin Plasma (n=5)	97-104%	92-101%	79-94%	89-97%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Complement Factor H Related Protein 5 (CFHR5). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Complement Factor H Related Protein 5 (CFHR5). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Complement Factor H Related Protein 5 (CFHR5), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Complement Factor H Related Protein 5 (CFHR5) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.312-20ng/mL
• ELISA Kit Recovery:

  Matrix	Recovery Range (%)	Average (%)
  Serum (n=5)	88-103	101
  EDTA Plasma (n=5)	88-99	95
  Heparin Plasma (n=5)	80-95	88

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Complement Factor H Related Protein 5 (CFHR5) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Complement Factor H Related Protein 5 (CFHR5) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-CFHR5 Microplate: 96 Wells (12 x 8 Well Strips)
  • CFHR5 Lyophilized Standard: 2 x 2 ng
  • 100X Biotinylated CFHR5 Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma and other biological fluids
• Sensitivity: < 14.8 pg/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Complement Factor H Related Protein 5 (CFHR5). No significant cross-reactivity or interference between Complement Factor H Related Protein 5 (CFHR5) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: CFHR5
• Gene Full Name: complement factor H related 5
• Alias Symbols: CFHL5;CFHR5D;complement factor H-related protein 5;factor H-related protein 5;FHR5;FHR-5.
• NCBI Gene Id: 81494
• Protein Name: Complement factor H-related protein 5
• Description of Target: Involved in complement regulation. The dimerized forms have avidity for tissue-bound complement fragments and efficiently compete with the physiological complement inhibitor CFH.
• Uniprot ID: Q9BXR6
• Protein Accession #: NP_110414.1
• Nucleotide Accession #: NM_030787.3.
• Protein Size (# AA): 569