Catalog No: OKCD09300
Size:96WELLS
Price: $750.00
SKU
OKCD09300
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Datasheets/ManualsPrintable datasheet for CFHR3 ELISA Kit (Human) (OKCD09300)
Product Info
Predicted Species ReactivityHomo sapiens|Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Complement Factor H Related Protein 3 (CFHR3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Complement Factor H Related Protein 3 (CFHR3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Complement Factor H Related Protein 3 (CFHR3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Complement Factor H Related Protein 3 (CFHR3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.156-10ng/mL
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Complement Factor H Related Protein 3 (CFHR3) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Complement Factor H Related Protein 3 (CFHR3) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
CFHR3 Microplate96 Wells (12 x 8 Well strips)
CFHR3 Lyophilized Standard2
100X CFHR3 HRP-Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 2 mL
Stop Solution1 x 20 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma and other biological fluids
Sensitivity< 0.075ng/mL
Gene SymbolCFHR3
Gene Full Namecomplement factor H related 3
Alias SymbolsCFHL3;complement factor H-related protein 3;DOWN16;FHR3;FHR-3;H factor-like 4;H factor-like protein 3;HLF4.
NCBI Gene Id10878
Protein NameComplement factor H-related protein 3
Description of TargetMight be involved in complement regulation.
Uniprot IDQ02985
Protein Accession #NP_001160096.1
Nucleotide Accession #NM_001166624.1
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