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Catalog No: OKCD02447
Size:96WELLS
Price: $650.00
SKU
OKCD02447
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Datasheets/ManualsPrintable datasheet for CFD ELISA Kit (Mouse) (OKCD02447)
Product Info
Predicted Species ReactivityMouse|Mus musculus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit Linearity
Sample1:21:41:81:16
Serum (n=5)78-101%88-97%80-96%95-103%
EDTA Plasma (n=5)97-105%94-103%89-101%87-94%
Heparin Plasma (n=5)96-103%80-94%80-105%89-96%
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Complement Factor D (CFD). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Complement Factor D (CFD). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Complement Factor D (CFD), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Complement Factor D (CFD) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.47-30ng/mL
ELISA Kit Recovery
MatrixRecovery range (%)Average(%)
Serum (n=5)78-9184
EDTA Plasma (n=5)78-9183
Heparin Plasma(n=5)94-10597
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Complement Factor D (CFD) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Complement Factor D (CFD) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-CFD Microplate96 Wells (12 x 8 Well strips)
CFD Lyophilized Standard2
100X Biotinylated CFD Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
Stop Solution1 x 6 mL
TMB Substrate1 x 9 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma, tissue homogenates, cell lysates, cell culture supernatant and other biological fluids
Sensitivity0.22 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Complement Factor D (CFD). No significant cross-reactivity or interference between Complement Factor D (CFD) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Protocol Information
Gene SymbolCfd
Gene Full Namecomplement factor D (adipsin)
Alias Symbols28 kDa adipocyte protein;A;Adipsin;Adn;C3 convertase activator;complement factor D;D component (adipsin) of complement;DF;properdin factor D.
NCBI Gene Id11537
Protein NameComplement factor D
Description of TargetFactor D cleaves factor B when the latter is complexed with factor C3b, activating the C3bbb complex, which then becomes the C3 convertase of the alternate pathway. Its function is homologous to that of C1s in the classical pathway.
Uniprot IDP03953
Protein Accession #NP_001278844.1
Nucleotide Accession #NM_001291915.2
Protein Size (# AA)259
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